Direct observation of motor protein stepping in living cells using MINFLUX

被引：71
|
作者：
Deguchi, Takahiro [1 ]
Iwanski, Malina K. [2 ]
Schentarra, Eva-Maria [1 ,3 ]
Heidebrecht, Christopher [1 ,3 ]
Schmidt, Lisa [1 ,3 ]
Heck, Jennifer [4 ]
Weihs, Tobias [5 ]
Schnorrenberg, Sebastian [6 ]
Hoess, Philipp [1 ]
Liu, Sheng [1 ,7 ]
Chevyreva, Veronika [1 ,8 ]
Noh, Kyung-Min [4 ]
Kapitein, Lukas C.
Ries, Jonas [1 ]
机构：
[1] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany
[2] Univ Utrecht, Fac Sci, Dept Biol, Cell Biol Neurobiol & Biophys, Utrecht, Netherlands
[3] Heidelberg Univ, Fac Biosci, Heidelberg, Germany
[4] European Mol Biol Lab, Genome Biol Unit, Heidelberg, Germany
[5] Abberior Instruments GmbH, Gottingen, Germany
[6] European Mol Biol Lab, EMBL Imaging Ctr, Heidelberg, Germany
[7] Univ New Mexico, Dept Phys & Astron, Albuquerque, NM USA
[8] FIRC Inst Mol Oncol, Milan, Italy
来源：
SCIENCE | 2023年 / 379卷 / 6636期
基金：
欧盟地平线“2020”; 欧洲研究理事会;
关键词：
TRACKING; RESOLUTION;
D O I：
10.1126/science.ade2676
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.
引用
收藏
页码：1010 / 1015
页数：6
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