Lipid biomarkers that reflect postoperative recurrence risk in lung cancer patients who smoke: a case-control study

被引：5

作者：

Takanashi, Yusuke ^{[1
]}

Kahyo, Tomoaki ^{[2
,3
]}

Hayakawa, Takamitsu ^{[1
]}

Sekihara, Keigo ^{[1
]}

Kawase, Akikazu ^{[1
]}

Kondo, Minako ^{[4
]}

Kitamoto, Takuya ^{[4
]}

Takahashi, Yutaka ^{[2
,3
]}

Sato, Tomohito ^{[2
]}

Sugimura, Haruhiko ^{[5
]}

Shiiya, Norihiko ^{[1
]}

Setou, Mitsutoshi ^{[2
,3
,6
]}

Funai, Kazuhito ^{[1
]}

机构：

[1] Hamamatsu Univ, Higashi Ward, Dept Surg 1, Sch Med, 1-20-1 Handayama, Hamamatsu, Shizuoka 4313192, Japan

[2] Hamamatsu Univ, Dept Cellular & Mol Anat, Sch Med, 1-20-1 Handayama,Higashi Ward, Hamamatsu, Shizuoka 4313192, Japan

[3] Hamamatsu Univ, Int Mass Imaging Ctr, Sch Med, 1-20-1 Handayama,Higashi Ward, Hamamatsu, Shizuoka 4313192, Japan

[4] Hamamatsu Univ, Adv Res Facil & Serv, Sch Med, 1-20-1 Handayama,Higashi Ward, Hamamatsu, Shizuoka 4313192, Japan

[5] Hamamatsu Univ, Dept Tumor Pathol, Sch Med, 1-20-1 Handayama,Higashi Ward, Hamamatsu, Shizuoka 4313192, Japan

[6] Hamamatsu Univ, Inst Med Photon Res, Dept Syst Mol Anat, Sch Med, 1-20-1 Handayama,Higashi Ward, Hamamatsu, Shizuoka 4313192, Japan

来源：

LIPIDS IN HEALTH AND DISEASE | 2023年 / 22卷 / 01期

关键词：

Lung cancer; Cigarette smoking; Recurrence risk; Lipid biomarker; Mass spectrometry; DESATURASE; 1; EXPRESSION; INTEROBSERVER AGREEMENT; TNM CLASSIFICATION; CELL; DROPLETS; MANAGEMENT; PREDICTOR; DIAGNOSIS; SURVIVAL; EDITION;

D O I：

10.1186/s12944-023-01778-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

BackgroundThe risk of postoperative recurrence is higher in lung cancer patients who smoke than non-smokers. However, objective evaluation of the postoperative recurrence risk is difficult using conventional pathological prognostic factors because of their lack of reproducibility. Consequently, novel objective biomarkers that reflect postoperative risk in lung cancer patients who smoke must be identified. Because cigarette smoking and oncogenesis alter lipid metabolism in lung tissue, we hypothesized that the lipid profiles in lung cancer tissues are influenced by cigarette smoking and can reflect the postoperative recurrence risk in smoking lung cancer patients. This study aimed to identify lipid biomarkers that reflect the smoking status and the postoperative recurrence risk.MethodsPrimary tumor tissues of lung adenocarcinoma (ADC) (n = 26) and squamous cell carcinoma (SQCC) (n = 18) obtained from surgery were assigned to subgroups according to the patient's smoking status. The ADC cohort was divided into never smoker and smoker groups, while the SQCC cohort was divided into moderate smoker and heavy smoker groups. Extracted lipids from the tumor tissues were subjected to liquid chromatography-tandem mass spectrometry analysis. Lipids that were influenced by smoking status and reflected postoperative recurrence and pathological prognostic factors were screened.ResultsTwo and 12 lipid peaks in the ADC and SQCC cohorts showed a significant positive correlation with the Brinkman index, respectively. Among them, in the ADC cohort, a higher lipid level consisted of three phosphatidylcholine (PC) isomers, PC (14:0_18:2), PC (16:1_16:1), and PC (16:0_16:2), was associated with a shorter recurrence free period (RFP) and a greater likelihoods of progressed T-factor (>= pT2) and pleural invasion. In the SQCC cohort, a lower m/z 736.5276 level was associated with shorter RFP and greater likelihood of recurrence.ConclusionsFrom our data, we propose three PC isomers, PC (14:0_18:2), PC (16:1_16:1), and PC (16:0_16:2), and a lipid peak of m/z 736.5276 as novel candidate biomarkers for postoperative recurrence risk in lung ADC and SQCC patients who are smokers.

引用

页数：13

共 44 条

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