Development of an Accessible and Scalable Quantitative Polymerase Chain Reaction Assay for Monkeypox Virus Detection

被引：9
作者：
Porzucek, Abigail J. [1 ]
Proctor, Abigale M. [1 ]
Klinkhammer, Katharina E. [1 ]
Tritsch, Sarah R. [1 ]
Robertson, Molly A. [1 ]
Bashor, Jonathan P. [1 ]
Villani, Jack [2 ]
Sepulveda, Jorge L. [2 ]
Mores, Christopher N. [1 ]
机构：
[1] George Washington Univ, Dept Global Hlth, Milken Inst Sch Publ Hlth, Washington, DC USA
[2] George Washington Univ, Sch Med & Hlth Sci, Publ Hlth Lab, Washington, DC 20052 USA
来源：
JOURNAL OF INFECTIOUS DISEASES | 2023年 / 227卷 / 09期
关键词：
PCR; diagnostics; emerging infectious disease; global health; monkeypox; outbreak response; qPCR assay;
D O I：
10.1093/infdis/jiac414
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
During the 2022 monkeypox (MPX) outbreak, testing has been limited and results delayed, allowing ongoing transmission. Gold-standard quantitative polymerase chain reaction (qPCR) diagnostics are difficult to obtain. This research adapted the June 2022 CDC MPX qPCR assay for broad implementation. Validated using MPX stocks in a matrix with multiple sample types, MPX was detected with cycle threshold (Ct) values 17.46-35.59 and titer equivalents 8.01 x 10(6) to 2.45 x 10(0) plaque-forming unit (PFU)/mL. The detection limit was 3.59 PFU/mL. Sensitivity and specificity were both 100%. This qPCR assay can be quickly and broadly implemented in research and public health laboratories to increase diagnostic capacity amid the growing MPX outbreak. Due to difficulty obtaining gold-standard qPCR diagnostics, this research adapted and validated the 2022 CDC monkeypox qPCR assay. This assay can be quickly and broadly implemented in research and public health laboratories to increase diagnostic capacity amid the growing outbreak.
引用
收藏
页码：1084 / 1087
页数：4
相关论文
共 50 条
[31]   A TaqMan quantitative polymerase chain reaction assay for the detection of Lawsonia intracellularis in fecal and tissue samples from pigs [J].
Richter, Barbara ;
Ladinig, Andrea ;
Nedorost, Nora ;
Weissenboeck, Herbert .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2010, 22 (01) :70-73
[32]   Development of polymerase chain reaction-lateral flow dipstick assay for detection of Mycoplasma bovis in cattle [J].
Song, Shengnan ;
Guo, Jia ;
Zhao, Yang ;
Shi, Feng ;
Wang, Yong ;
Zhang, Qian ;
Wang, Zhen ;
Chen, Chuangfu .
BMC VETERINARY RESEARCH, 2024, 20 (01)
[33]   Real-time fluorogenic reverse transcription polymerase chain reaction assay for the specific detection of Bagaza virus [J].
Buitrago, Dolores ;
Rocha, Ana ;
Tena-Tomas, Cristina ;
Vigo, Marta ;
Agueero, Montserrat ;
Angel Jimenez-Clavero, Miguel .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2012, 24 (05) :959-963
[34]   Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice [J].
Wang, K. W. ;
Chueh, L. L. ;
Wang, M. H. ;
Huang, Y. T. ;
Fang, B. H. ;
Chang, C. Y. ;
Fang, M. C. ;
Chou, J. Y. ;
Hsieh, S. C. ;
Wan, C. H. .
LABORATORY ANIMALS, 2013, 47 (02) :116-121
[35]   Evaluation of the analytical sensitivity of a polymerase chain reaction assay for the detection of chicken infectious anemia virus in avian vaccines [J].
Hermann, J. ;
Koski, D. ;
Taylor, S. ;
Gatewood, D. .
BIOLOGICALS, 2012, 40 (04) :266-269
[36]   A miniaturized quantitative polymerase chain reaction system for DNA amplification and detection [J].
Wang, Jung-Hao ;
Chien, Liang-Ju ;
Hsieh, Tsung-Min ;
Luo, Ching-Hsing ;
Chou, Wen-Pin ;
Chen, Ping-Hei ;
Chen, Pei-Jer ;
Lee, Da-Sheng ;
Lee, Gwo-Bin .
SENSORS AND ACTUATORS B-CHEMICAL, 2009, 141 (01) :329-337
[37]   Micro-droplet Digital Polymerase Chain Reaction and Real-Time Quantitative Polymerase Chain Reaction Technologies Provide Highly Sensitive and Accurate Detection of Zika Virus [J].
Hui, Yuan ;
Wu, Zhiming ;
Qin, Zhiran ;
Zhu, Li ;
Liang, Junhe ;
Li, Xujuan ;
Fu, Hanmin ;
Feng, Shiyu ;
Yu, Jianhai ;
He, Xiaoen ;
Lu, Weizhi ;
Xiao, Weiwei ;
Wu, Qinghua ;
Zhang, Bao ;
Zhao, Wei .
VIROLOGICA SINICA, 2018, 33 (03) :270-277
[38]   Development of a triplex quantitative reverse transcription-polymerase chain reaction for the detection of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, and porcine rotavirus A [J].
Luo, Tingyu ;
Li, Kaili ;
Li, Changwen ;
Xia, Changyou ;
Gao, Caixia .
FRONTIERS IN MICROBIOLOGY, 2024, 15
[39]   Detection of Rabies Virus Genes by In-Situ Polymerase Chain Reaction [J].
P. Ezhil Praveena ;
R. Jayakumar ;
C. Balachandran ;
G. Thirumurugan ;
G. Dhinakar Raj ;
B. Murali Manohar .
Veterinary Research Communications, 2007, 31 :775-781
[40]   Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction [J].
Spiegel, S ;
Scott, SW ;
BowmanVance, V ;
Tam, Y ;
Galiakparov, NN ;
Rosner, A .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 1996, 102 (07) :681-685
12345