Development of an Accessible and Scalable Quantitative Polymerase Chain Reaction Assay for Monkeypox Virus Detection

被引：10
作者：
Porzucek, Abigail J. [1 ]
Proctor, Abigale M. [1 ]
Klinkhammer, Katharina E. [1 ]
Tritsch, Sarah R. [1 ]
Robertson, Molly A. [1 ]
Bashor, Jonathan P. [1 ]
Villani, Jack [2 ]
Sepulveda, Jorge L. [2 ]
Mores, Christopher N. [1 ]
机构：
[1] George Washington Univ, Dept Global Hlth, Milken Inst Sch Publ Hlth, Washington, DC USA
[2] George Washington Univ, Sch Med & Hlth Sci, Publ Hlth Lab, Washington, DC 20052 USA
来源：
JOURNAL OF INFECTIOUS DISEASES | 2023年 / 227卷 / 09期
关键词：
PCR; diagnostics; emerging infectious disease; global health; monkeypox; outbreak response; qPCR assay;
D O I：
10.1093/infdis/jiac414
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
During the 2022 monkeypox (MPX) outbreak, testing has been limited and results delayed, allowing ongoing transmission. Gold-standard quantitative polymerase chain reaction (qPCR) diagnostics are difficult to obtain. This research adapted the June 2022 CDC MPX qPCR assay for broad implementation. Validated using MPX stocks in a matrix with multiple sample types, MPX was detected with cycle threshold (Ct) values 17.46-35.59 and titer equivalents 8.01 x 10(6) to 2.45 x 10(0) plaque-forming unit (PFU)/mL. The detection limit was 3.59 PFU/mL. Sensitivity and specificity were both 100%. This qPCR assay can be quickly and broadly implemented in research and public health laboratories to increase diagnostic capacity amid the growing MPX outbreak. Due to difficulty obtaining gold-standard qPCR diagnostics, this research adapted and validated the 2022 CDC monkeypox qPCR assay. This assay can be quickly and broadly implemented in research and public health laboratories to increase diagnostic capacity amid the growing outbreak.
引用
收藏
页码：1084 / 1087
页数：4
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