tRNA engineering strategies for genetic code expansion

被引：11
作者：
Kim, Youjin [1 ]
Cho, Suho [1 ]
Kim, Joo-Chan [1 ]
Park, Hee-Sung [1 ]
机构：
[1] Korea Adv Inst Sci & Technol, Dept Chem, Daejeon, South Korea
来源：
FRONTIERS IN GENETICS | 2024年 / 15卷
基金：
新加坡国家研究基金会;
关键词：
tRNA engineering; genetic code expansion; directed evolution; rational design; unnatural amino acid; UNNATURAL AMINO-ACIDS; SITE-SPECIFIC INCORPORATION; SUPPRESSOR TRANSFER-RNAS; ESCHERICHIA-COLI; IN-VIVO; NONNATURAL RESIDUES; DIRECTED-EVOLUTION; CRYSTAL-STRUCTURE; AMBER SUPPRESSOR; SYNTHETASE PAIR;
D O I：
10.3389/fgene.2024.1373250
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The advancement of genetic code expansion (GCE) technology is attributed to the establishment of specific aminoacyl-tRNA synthetase/tRNA pairs. While earlier improvements mainly focused on aminoacyl-tRNA synthetases, recent studies have highlighted the importance of optimizing tRNA sequences to enhance both unnatural amino acid incorporation efficiency and orthogonality. Given the crucial role of tRNAs in the translation process and their substantial impact on overall GCE efficiency, ongoing efforts are dedicated to the development of tRNA engineering techniques. This review explores diverse tRNA engineering approaches and provides illustrative examples in the context of GCE, offering insights into the user-friendly implementation of GCE technology.
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页数：13
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