pH-sensing supramolecular fluorescent probes discovered by library screening

被引：5
作者：
Yang, Jingyu [1 ]
Zhao, Zizhen [1 ]
Jiang, Siyang [1 ]
Zhang, Lingyu [1 ]
Zhao, Kai [3 ,4 ]
Li, Zhan-Ting [1 ]
Ma, Da [2 ,3 ]
机构：
[1] Fudan Univ, Dept Chem, Shanghai 200438, Peoples R China
[2] Taizhou Univ, Sch Pharmaceut Engn, Jiaojiang 318000, Zhejiang, Peoples R China
[3] Taizhou Univ, Inst Adv Studies, Jiaojiang 318000, Zhejiang, Peoples R China
[4] Taizhou Univ, Sch Life Sci, Jiaojiang 318000, Zhejiang, Peoples R China
来源：
TALANTA | 2023年 / 263卷
基金：
中国国家自然科学基金;
关键词：
Fluorescent probe; pH sensing; Host -guest chemistry; Library screening; Cellular detection; INTRACELLULAR PH; DYES;
D O I：
10.1016/j.talanta.2023.124716
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new design concept for pH-sensing supramolecular fluorescent probes is reported. Supramolecular fluorescent pH probes based on pro-guest are designed and prepared. Pro-guests are designed to degrade under acidic condition and convert to competitive guests to displace encapsulated dyes, which leads to a significant enhancement in fluorescence intensity. A library of potential fluorescent pH probes is generated and screened to discover workable probes. These probes are capable of detecting the acidic pH in solution phase. We confirm that these supramolecular probes could detect the acidic environment in endosomal compartments in live cells.
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页数：7
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