Antiproliferative Potential of Dypsis decaryi Seeds Supported by Metabolic Profiling and Molecular Docking

Introduction: Dypsis decaryi seeds need more phytochemical and biological attention specially on one of the most important life -threatening problems (cancer). Methods: Total ethanol extract of D. decaryi seeds (TEEDDS, Arecaceae) and its fixed oil fraction (FOF) were subjected to UPLC-HR-ESI-MS metabolic profiling and gas chromatography -mass spectrometry, respectively. Also, TEEDDS, FOF, and FOF silver nanoparticles (FOF-SNPs) were screened using 4 cell lines. FOF effects on expression of Bcl-2, Bax, P53, and epidermal growth factor receptor (EGFR) in human prostate carcinoma cell line (PC -3) were evaluated and supported by docking study. Results: Metabolic profiling of TEEDDS resulted in identification of 18 compounds. While GC -MS of FOF displayed the presence of 12 fatty acids methyl esters. TEEDDS, staurosporine, FOF, and FOF-SNPs showed IC50= 3.55, 9.81, 16.88, and 0.01 mu g/mL, respectively, against PC -3 (human prostate carcinoma cell line). FOF effects on the expression of Bcl-2, Bax, P53, and EGFR in PC -3 cell lines displayed IC50= 1.09, 220.6, 619.8, and 399.64 ng/mL, respectively. The FOF major constituent (E-13-octadecenoic acid) exhibited significant affinity and binding interactions that were comparable with that of the cocrystalized inhibitor. Discussion/Conclusions: For the first time, in vitro antiproliferative activity against PC -3, HepG-2, HCT-116, and MCF-7 cells, and chemical profiles of TEEDDS and its FOF have been noteworthy studied. FOF and FOF-SNPs displayed the highest activities particularly on PC -3. Additionally, FOF showed significant activities on Bcl-2, Bax, P53, and EGFR, which was also supported by docking study. These results highlight that FOF and FOF-SNPs could have potent antiproliferative activity against PC -3 cell lines and may play an important role in antiproliferative drug development.