ASu@MNPs-based electrochemical immunosensor for vitamin D3 serum samples analysis

被引：17

作者：

Polli, Francesca ^{[1
]}

D'Agostino, Cristine ^{[1
]}

Zumpano, Rosaceleste ^{[1
]}

De Martino, Viviana ^{[2
]}

Favero, Gabriele ^{[3
]}

Colangelo, Luciano ^{[2
]}

Minisola, Salvatore ^{[2
]}

Mazzei, Franco ^{[1
]}

机构：

[1] Sapienza Univ Rome, Dept Chem & Drug Technol, Ple Aldo Moro 5, I-00185 Rome, Italy

[2] Sapienza Univ Rome, Dept Clin Internal Anaesthesiol & Cardiovasc Sci, Ple Aldo Moro 5, I-00185 Rome, Italy

[3] Sapienza Univ Rome, Dept Environm Biol, Ple Aldo Moro 5, I-00185 Rome, Italy

来源：

TALANTA | 2023年 / 251卷

关键词：

Vitamin D-3 detection; Gold -magnetic nanoparticles; Label -free immunosensor; Differential pulse voltammetry; BIOSENSING PLATFORM; NANOPARTICLES; GLUTARALDEHYDE; ADSORPTION; SURFACE;

D O I：

10.1016/j.talanta.2022.123755

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a new sensitive label-free electrochemical immunosensor to detect Vitamin D-3 (25-OHD3) in untreated serum samples. To this aim, a graphite screen printed electrode (SPE) was modified using cysteamine (CYM) functionalized core-shell magnetic nanoparticles (Au@MNPs) then, the 25-OHD3 antibody (AbD) was immobilized via glutaraldehyde crosslinking. The several steps involved in the immunosensor development and 25-OHD3 analysis were monitored by using differential pulse voltammetry (DPV). The developed immunosensor showed a LOD of 2.4 ng mL(-1) and a linear range between 7.4 and 70 ng mL(-1). The effectiveness of the immunosensor in human serum analysis was assessed by comparing the results obtained with the chemiluminescence-immunoassay (CLIA) reference method. The high sensitivity and excellent agreement with the reference method suggest its potential use as a POCT to monitor hypovitaminosis 25-OHD levels.

引用

页数：8

共 43 条

[11] Electrochemical immunosensor based on magnetite nanoparticles incorporated electrospun polyacrylonitrile nanofibers for Vitamin-D3 detection [J].

Chauhan, Deepika ;

Gupta, Pramod K. ;

Solanki, Pratima R. .

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2018, 93 :145-156

[12]

Chen LG, 2015, ANAL METHODS-UK, V7, P2354, DOI [10.1039/c4ay01981d, 10.1039/C4AY01981D]

[13] MECHANISM OF CROSSLINKING OF PROTEINS BY GLUTARALDEHYDE .3. REACTION WITH COLLAGEN IN TISSUES [J].

CHEUNG, DT ;

PERELMAN, N ;

KO, EC ;

NIMNI, ME .

CONNECTIVE TISSUE RESEARCH, 1985, 13 (02) :109-115

[14] Simulation of Physicochemical and Pharmacokinetic Properties of Vitamin D3and Its Natural Derivatives [J].

Deb, Subrata ;

Reeves, Anthony Allen ;

Lafortune, Suki .

PHARMACEUTICALS, 2020, 13 (08) :1-16

[15] Method evaluation study of a new generation of vitamin D assays [J].

Enko, Dietmar ;

Kriegshaeuser, Gernot ;

Stolba, Robert ;

Worf, Elfriede ;

Halwachs-Baumann, Gabriele .

BIOCHEMIA MEDICA, 2015, 25 (02) :203-212

[16] Step-growth thiol-thiol photopolymerization as radiation curing technology [J].

Feillee, Noemi ;

De Fina, Maurizio ;

Ponche, Arnaud ;

Vaulot, Cyril ;

Rigolet, Severinne ;

Jacomine, Leandro ;

Majjad, Hicham ;

Ley, Christian ;

Chemtob, Abraham .

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2017, 55 (01) :117-128

[17] The relative influence of serum ionized calcium and 25-hydroxyvitamin D in regulating PTH secretion in healthy subjects [J].

Ferrone, Federica ;

Pepe, Jessica ;

Danese, Vittoria Carmela ;

Fassino, Valeria ;

Cecchetti, Veronica ;

De Lucia, Federica ;

Biamonte, Federica ;

Colangelo, Luciano ;

Ferrazza, Giancarlo ;

Panzini, Enrico ;

Scillitani, Alfredo ;

Nieddu, Luciano ;

Blocki, Frank ;

Rao, Sudhaker D. ;

Minisola, Salvatore ;

Cipriani, Cristiana .

BONE, 2019, 125 :200-206

[18] Hydrophobic and Hydrophilic Au and Ag Nanoparticles. Breakthroughs and Perspectives [J].

Fratoddi, Ilaria .

NANOMATERIALS, 2018, 8 (01)

[19] Direct electrical control of IgG conformation and functional activity at surfaces [J].

Ghisellini, Paola ;

Caiazzo, Marialuisa ;

Alessandrini, Andrea ;

Eggenhoffner, Roberto ;

Vassalli, Massimo ;

Facci, Paolo .

SCIENTIFIC REPORTS, 2016, 6

[20] Controversies in Vitamin D: A Statement From the Third International Conference [J].

Giustina, Andrea ;

Bouillon, Roger ;

Binkley, Neil ;

Sempos, Christopher ;

Adler, Robert A. ;

Bollerslev, Jens ;

Dawson-Hughes, Bess ;

Ebeling, Peter R. ;

Feldman, David ;

Heijboer, Annemieke ;

Jones, Glenville ;

Kovacs, Christopher S. ;

Lazaretti-Castro, Marise ;

Lips, Paul ;

Marcocci, Claudio ;

Minisola, Salvatore ;

Napoli, Nicola ;

Rizzoli, Rene ;

Scragg, Robert ;

White, John H. ;

Formenti, Anna Maria ;

Bilezikian, John P. .

JBMR PLUS, 2020, 4 (12)

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