ASu@MNPs-based electrochemical immunosensor for vitamin D3 serum samples analysis

被引：17

作者：

Polli, Francesca ^{[1
]}

D'Agostino, Cristine ^{[1
]}

Zumpano, Rosaceleste ^{[1
]}

De Martino, Viviana ^{[2
]}

Favero, Gabriele ^{[3
]}

Colangelo, Luciano ^{[2
]}

Minisola, Salvatore ^{[2
]}

Mazzei, Franco ^{[1
]}

机构：

[1] Sapienza Univ Rome, Dept Chem & Drug Technol, Ple Aldo Moro 5, I-00185 Rome, Italy

[2] Sapienza Univ Rome, Dept Clin Internal Anaesthesiol & Cardiovasc Sci, Ple Aldo Moro 5, I-00185 Rome, Italy

[3] Sapienza Univ Rome, Dept Environm Biol, Ple Aldo Moro 5, I-00185 Rome, Italy

来源：

TALANTA | 2023年 / 251卷

关键词：

Vitamin D-3 detection; Gold -magnetic nanoparticles; Label -free immunosensor; Differential pulse voltammetry; BIOSENSING PLATFORM; NANOPARTICLES; GLUTARALDEHYDE; ADSORPTION; SURFACE;

D O I：

10.1016/j.talanta.2022.123755

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a new sensitive label-free electrochemical immunosensor to detect Vitamin D-3 (25-OHD3) in untreated serum samples. To this aim, a graphite screen printed electrode (SPE) was modified using cysteamine (CYM) functionalized core-shell magnetic nanoparticles (Au@MNPs) then, the 25-OHD3 antibody (AbD) was immobilized via glutaraldehyde crosslinking. The several steps involved in the immunosensor development and 25-OHD3 analysis were monitored by using differential pulse voltammetry (DPV). The developed immunosensor showed a LOD of 2.4 ng mL(-1) and a linear range between 7.4 and 70 ng mL(-1). The effectiveness of the immunosensor in human serum analysis was assessed by comparing the results obtained with the chemiluminescence-immunoassay (CLIA) reference method. The high sensitivity and excellent agreement with the reference method suggest its potential use as a POCT to monitor hypovitaminosis 25-OHD levels.

引用

页数：8

共 43 条

[1] pH-Responsive Mercaptoundecanoic Acid Functionalized Gold Nanoparticles and Applications in Catalysis [J].

Ansar, Siyam M. ;

Chakraborty, Saptarshi ;

Kitchens, Christopher L. .

NANOMATERIALS, 2018, 8 (05)

[2] Extra-Skeletal Effects of Vitamin D [J].

Bouillon, Roger .

VITAMIN D IN CLINICAL MEDICINE, 2018, 50 :72-88

[3] ADSORPTION OF MONOCLONAL IGGS AND THEIR F(AB')(2) FRAGMENTS ONTO POLYMERIC SURFACES [J].

BUIJS, J ;

LICHTENBELT, JWT ;

NORDE, W ;

LYKLEMA, J .

COLLOIDS AND SURFACES B-BIOINTERFACES, 1995, 5 (1-2) :11-23

[4] Synthesis and characterization of α-nickel (II) hydroxide particles on organic-inorganic matrix and its application in a sensitive electrochemical sensor for vitamin D determination [J].

Canevari, Thiago C. ;

Cincotto, Fernando H. ;

Landers, Richard ;

Machado, Sergio A. S. .

ELECTROCHIMICA ACTA, 2014, 147 :688-695

[5] Lignin nanoparticles are renewable and functional platforms for the concanavalin a oriented immobilization of glucose oxidase-peroxidase in cascade bio-sensing [J].

Capecchi, Eliana ;

Piccinino, Davide ;

Tomaino, Elisabetta ;

Bizzarri, Bruno Mattia ;

Polli, Francesca ;

Antiochia, Riccarda ;

Mazzei, Franco ;

Saladino, Raffaele .

RSC ADVANCES, 2020, 10 (48) :29031-29042

[6] Several approaches for vitamin D determination by surface plasmon resonance and electrochemical affinity biosensors [J].

Carlucci, Luciano ;

Favero, Gabriele ;

Tortolini, Cristina ;

Di Fusco, Massimo ;

Romagnoli, Elisabetta ;

Minisola, Salvatore ;

Mazzei, Franco .

BIOSENSORS & BIOELECTRONICS, 2013, 40 (01) :350-355

[7] Amine Functionalized Noble Metal: Metal Oxide Nanohybrid for Efficient Electrochemical Determination of 25-Hydroxy Vitamin-D3 in Human Serum [J].

Chauhan, Deepika ;

Gupta, Pramod K. ;

Solanki, Pratima R. .

JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2021, 168 (11)

[8] Carbon cloth-based immunosensor for detection of 25-hydroxy vitamin D3 [J].

Chauhan, Deepika ;

Yadav, Amit K. ;

Solanki, Pratima R. .

MICROCHIMICA ACTA, 2021, 188 (04)

[9] An efficient electrochemical biosensor for Vitamin-D3 detection based on aspartic acid functionalized gadolinium oxide nanorods [J].

Chauhan, Deepika ;

Kumar, Robin ;

Panda, Amulya K. ;

Solanki, Pratima R. .

JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T, 2019, 8 (06) :5490-5503

[10] Hydrophilic and Insoluble Electrospun Cellulose Acetate Fiber-Based Biosensing Platform for 25-Hydroxy Vitamin-D3 Detection [J].

Chauhan, Deepika ;

Solanki, Pratima R. .

ACS APPLIED POLYMER MATERIALS, 2019, 1 (07) :1613-1623

← 1 2 3 4 5 →