Identification of laccase genes in Trichoderma asperellum Ts93

被引:4
作者
Fu, Kehe [1 ]
Fan, Lili [1 ]
Luo, Xin [1 ]
机构
[1] Nanchang Normal Univ, Dept Life Sci, RuiXiang Rd 889, Nanchang 330032, Jiangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Genome-wide; Laccase; Optimization of culture conditions; Trichoderma spp; FUNGAL LACCASES; OPTIMIZATION; DEGRADATION;
D O I
10.1007/s10529-023-03349-z
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Although extensive research efforts have been dedicated to characterizing the laccase from white-rot fungus, little information is available on laccases from Trichoderma spp. A copper-tolerant strain with the ability to produce laccase was isolated and identified as Trichoderma asperellum Ts93. Under optimized conditions, the maximum laccase activity was 1.96 U/ml. The genome-wide survey of Ts93 revealed the presence of seven putative laccase genes that all contained the conserved domain and were divided into three different phylogenetic groups. Among these genes, three contained the four copper-binding conserved regions. The expression profiles acquired through real-time quantitative PCR analysis showed that five of the seven genes were significantly upregulated in response to laccase activity. Seven laccase genes in T. asperellum were identified for the first time by whole-genome sequencing followed by phylogenetic analysis. The findings of this work provide valuable information for the functional analysis of laccase genes in Trichoderma spp.
引用
收藏
页码:479 / 487
页数:9
相关论文
共 20 条
[1]   Comparative Study on Laccase Activity of White Rot Fungi under Submerged Fermentation with Different Lignocellulosic Wastes [J].
An, Qi ;
Qiao, Jie ;
Bian, Lu-Sen ;
Han, Mei-Ling ;
Yan, Xun-You ;
Liu, Zi-Zheng ;
Xie, Chun-Yan .
BIORESOURCES, 2020, 15 (04) :9166-9179
[2]   Fungal laccases - occurrence and properties [J].
Baldrian, P .
FEMS MICROBIOLOGY REVIEWS, 2006, 30 (02) :215-242
[3]   Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions [J].
Bamforth, SM ;
Singleton, I .
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2005, 80 (07) :723-736
[4]   Hazardous contaminants in the environment and their laccase-assisted degradation - A review [J].
Bilal, Muhammad ;
Rasheed, Tahir ;
Nabeel, Faran ;
Iqbal, Hafiz M. N. ;
Zhao, Yuping .
JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2019, 234 :253-264
[5]   Trichoderma: the genomics of opportunistic success [J].
Druzhinina, Irina S. ;
Seidl-Seiboth, Verena ;
Herrera-Estrella, Alfredo ;
Horwitz, Benjamin A. ;
Kenerley, Charles M. ;
Monte, Enrique ;
Mukherjee, Prasun K. ;
Zeilinger, Susanne ;
Grigoriev, Igor V. ;
Kubicek, Christian P. .
NATURE REVIEWS MICROBIOLOGY, 2011, 9 (10) :749-759
[6]   Physiological regulation of laccase and manganese peroxidase production by white-rot Basidiomycetes [J].
Elisashvili, Vladimir ;
Kachlishvili, Eva .
JOURNAL OF BIOTECHNOLOGY, 2009, 144 (01) :37-42
[7]   Laccases: a never-ending story [J].
Giardina, Paola ;
Faraco, Vincenza ;
Pezzella, Cinzia ;
Piscitelli, Alessandra ;
Vanhulle, Sophie ;
Sannia, Giovanni .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2010, 67 (03) :369-385
[8]   Novel insight into adsorption and co-adsorption of heavy metal ions and an organic pollutant by magnetic graphene nanomaterials in water [J].
Huang, Dan ;
Wu, Jizi ;
Wang, Lu ;
Liu, Xingmei ;
Meng, Jun ;
Tang, Xianjin ;
Tang, Caixian ;
Xu, Jianming .
CHEMICAL ENGINEERING JOURNAL, 2019, 358 :1399-1409
[9]   Optimization of Laccase Production by Locally Isolated Trichoderma muroiana IS1037 Using Rubber Wood Dust as Substrate [J].
Jaber, Saja Mahdey ;
Shah, Umi Kalsom Md ;
Asa'ari, Ainun Zuriyati Mohamed ;
Ariff, Arbakariya B. .
BIORESOURCES, 2017, 12 (02) :3834-3849
[10]   Laccase catalysis for the synthesis of bioactive compounds [J].
Kudanga, Tukayi ;
Nemadziva, Blessing ;
Le Roes-Hill, Marilize .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101 (01) :13-33