Insights into Metabolic Engineering of Bioactive Molecules in Tetrastigma hemsleyanum Diels & Gilg: A Traditional Medicinal Herb

被引:2
作者
Krishna, T. P. Ajeesh [1 ]
Maharajan, T. [1 ]
Krishna, T. P. Adarsh [2 ]
Ceasar, S. Antony [1 ]
机构
[1] Rajagiri Coll Social Sci, Dept Biosci, Div Plant Mol Biol & Biotechnol, Kochi 683104, Kerala, India
[2] Sreedhareeyam Farmherbs India Pvt Ltd, Res & Dev Div, Ernakulam 686662, Kerala, India
关键词
Drug discovery; genome editing; flavanoids; metabolic engineering; Tetrastigma hemsleyanum; bioactive molecules; PYRROLIZIDINE ALKALOIDS; ANTIOXIDANT ACTIVITY; SECONDARY METABOLITES; TARGETED MUTAGENESIS; SUSPENSION-CULTURES; ANTITUMOR-ACTIVITY; RADIX-TETRASTIGMA; CALLUS-CULTURES; GENOME; BIOSYNTHESIS;
D O I
10.2174/0113892029251472230921053135
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plants are a vital source of bioactive molecules for various drug development processes. Tetrastigma hemsleyanum is one of the endangered medicinal plant species well known to the world due to its wide range of therapeutic effects. Many bioactive molecules have been identified from this plant, including many classes of secondary metabolites such as flavonoids, phenols, terpenoids, steroids, alkaloids, etc. Due to its slow growth, it usually takes 3-5 years to meet commercial medicinal materials for this plant. Also, T. hemsleyanum contains low amounts of specific bioactive compounds, which are challenging to isolate easily. Currently, scientists are attempting to increase bioactive molecules' production from medicinal plants in different ways or to synthesize them chemically. The genomic tools helped to understand medicinal plants' genome organization and led to manipulating genes responsible for various biosynthesis pathways. Metabolic engineering has made it possible to enhance the production of secondary metabolites by introducing manipulated biosynthetic pathways to attain high levels of desirable bioactive molecules. Metabolic engineering is a promising approach for improving the production of secondary metabolites over a short time period. In this review, we have highlighted the scope of various biotechnological approaches for metabolic engineering to enhance the production of secondary metabolites for pharmaceutical applications in T. hemsleyanum. Also, we summarized the progress made in metabolic engineering for bioactive molecule enhancement in T. hemsleyanum. It may lead to reducing the destruction of the natural habitat of T. hemsleyanum and conserving them through the cost-effective production of bioactive molecules in the future.
引用
收藏
页码:72 / 83
页数:12
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