Study of the antioxidant and anti-pancreatic cancer activities of Anchusa strigosa aqueous extracts obtained by maceration and ultrasonic extraction techniques

被引:3
作者
Chebaro, Ziad [1 ]
Abdallah, Rola [2 ]
Badran, Adnan [3 ]
Hamade, Kamar [4 ]
Hijazi, Akram [1 ]
Maresca, Marc [5 ]
Mesmar, Joelle Edward [2 ]
Baydoun, Elias [2 ]
机构
[1] Platforme Rech & Anal Sci Environm EDST PRASE, Beirut, Lebanon
[2] Amer Univ Beirut, Dept Biol, Beirut, Lebanon
[3] Univ Petra, Dept Nutr, Amman, Jordan
[4] Univ Picardie Jules Verne, Lab BIOPI, UMRT INRE 1158 BioEcoAgro, Amiens, France
[5] Aix Marseille Univ, CNRS, Cent Marseille, iSM2, Marseille, France
关键词
Anchusa strigosa; pancreatic cancer; herbal medicine; bioactive compounds; conventional extraction; ultrasound-assisted extraction; apoptosis; COX-2; PYRROLIZIDINE ALKALOIDS; MEDICINAL-PLANTS;
D O I
10.3389/fphar.2023.1201969
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Pancreatic cancer is a highly aggressive malignancy and a leading cause of cancerrelated deaths worldwide. Moreover, the incidence and mortality rates for pancreatic cancer are projected to keep increasing. A major challenge in the treatment of pancreatic cancer is the lack of effective screening approaches, which contributes to its poor prognosis, indicating the need for new treatment regimens and alternative therapies, such as herbal medicine. The medicinal plant A. strigosa, which is widely distributed in the Eastern Mediterranean region, is a short prickly plant from the Boraginaceae family that has been widely used in traditional medicine for treating various diseases. Nevertheless, its effect on human pancreatic cancer remains poorly investigated. In the present study, we screened the phytochemical content of Anchusa strigosa aqueous extracts obtained by maceration and ultrasound-assisted methods (ASM and ASU, respectively) and evaluated their antioxidant effects. We also investigated their anticancer effects and possible underlying mechanisms. The results show that both extracts were rich in bioactive molecules, with slight differences in their composition. Both extracts exhibited remarkable antioxidant potential and potent radical-scavenging activity in vitro. Additionally, non-cytotoxic concentrations of both extracts attenuated cell proliferation in a time- and concentrationdependent manner, which was associated with a decrease in the proliferation marker Ki67 and an induction of the intrinsic apoptotic pathway. Furthermore, the extracts increased the aggregation of pancreatic cancer cells and reduced their migratory potential, with a concomitant downregulation of integrin beta 1. Finally, we showed that the ASM extract caused a significant decrease in the levels of COX-2, an enzyme that has been linked to inflammation, carcinogenesis, tumor progression, and metastasis. Taken together, our findings provide evidence that A. strigosa extracts, particularly the extract obtained using the maceration method, have a potential anticancer effect and may represent a new resource for the design of novel drugs against pancreatic cancer.
引用
收藏
页数:15
相关论文
共 75 条
[1]   Syringic acid from Tamarix aucheriana possesses antimitogenic and chemo-sensitizing activities in human colorectal cancer cells [J].
Abaza, Mohamed-Salah ;
Al-Attiyah, Raja'a ;
Bhardwaj, Radhika ;
Abbadi, Ghaneim ;
Koyippally, Mathew ;
Afzal, Mohammad .
PHARMACEUTICAL BIOLOGY, 2013, 51 (09) :1110-1124
[2]  
Abbas M., 2009, JORDAN J PHARM SCI, V2, P131139
[3]  
Abdulla D. N., 2022, INT SCI C PUR, P372
[4]  
Abu-Rabia A., 2015, Indigenous Medicine Among the Bedouin in the Middle East
[5]   Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes [J].
Abubakar, Abdullahi R. ;
Haque, Mainul .
JOURNAL OF PHARMACY AND BIOALLIED SCIENCES, 2020, 12 (01) :1-10
[6]   Pepsin inhibitor from roots of Anchusa strigosa [J].
Abuereish, GM .
PHYTOCHEMISTRY, 1998, 48 (02) :217-221
[7]  
Abutbul S, 2005, ISR J AQUACULT-BAMID, V57, P71
[8]   A survey of medicinal plants and their traditional uses in Iraq [J].
Al-douri, NA .
PHARMACEUTICAL BIOLOGY, 2000, 38 (01) :74-79
[9]   Antioxidant Potential of Pistacia vera L. fruit hull, Anchusa strigosa flowers and Llex paraguariensis A. St.-Hil. leaves extract [J].
Al-Khateeb, Ekbal Hasan ;
Al-Assi, Ghada Ahmad ;
Shakya, Ashok K. ;
Al-Rawi, Naseer ;
Shalan, Naeem .
ORIENTAL JOURNAL OF CHEMISTRY, 2019, 35 (03) :982-986
[10]   The Cytotoxic Effect of the Extract of Anchusa strigosa (Him Him) Grown in Jordan Against Different Cancer Cell Lines [J].
Al-Khatib, Arwa ;
Al-Khateeb, Iqbal ;
Abu-Dahab, Rana ;
Al-Rawi, Naseer .
BAGHDAD SCIENCE JOURNAL, 2021, 18 (01) :70-76