Apoptosis induction in human hepatoma cell line HepG2 cells by trans-Anethole via activation of mitochondria-mediated apoptotic pathways

被引：6

作者：

Harakeh, Steve ^{[1
,2
]}

Al-Raddadi, Rajaa ^{[3
]}

Alamri, Turki ^{[4
]}

Al-Jaouni, Soad ^{[2
,5
]}

Qari, Mohammed ^{[2
,5
]}

Qari, Yousef ^{[6
]}

Kumar, Ajay ^{[7
]}

Tashkandi, Hanaa M. ^{[8
]}

Moulay, Mohammed ^{[9
,10
]}

Aldahlawi, Alia M. ^{[11
,12
]}

Slama, Petr ^{[13
]}

Haque, Shafiul ^{[14
,15
,16
]}

机构：

[1] King Abdulaziz Univ, King Fahd Med Res Ctr, Jeddah 21589, Saudi Arabia

[2] King Abdulaziz Univ, Fac Med, Yousef Abdul Latif Jameel Sci Chair Prophet Med Ap, Jeddah 21589, Saudi Arabia

[3] King Abdulaziz Univ, Fac Med, Dept Community Med, Jeddah 21589, Saudi Arabia

[4] King Abdulaziz Univ, Fac Med Rabigh, Family & Community Med Dept, Jeddah, Saudi Arabia

[5] King Abdulaziz Univ, King Abdulaziz Univ Hosp, Fac Med, Dept Hematol Pediat Oncol, Jeddah 21589, Saudi Arabia

[6] King Abdulaziz Univ, Fac Med, Dept Med, Jeddah, Saudi Arabia

[7] Chandigarh Univ, Univ Ctr Res & Dev, Biotechnol Engn & Food Technol, Mohali 140413, Punjab, India

[8] King Abdulaziz Univ, Fac Med, Dept Gen Surg, Jeddah 21589, Saudi Arabia

[9] King Abdulaziz Univ, King Fahd Med Res Ctr, Fac Appl Med Sci, Dept Med Lab Sci, Jeddah 21589, Saudi Arabia

[10] King Abdulaziz Univ, Fac Appl Med Sci, Dept Med Lab Sci, Jeddah 21589, Saudi Arabia

[11] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah, Saudi Arabia

[12] King Abdulaziz Univ, King Fahd Med Res Ctr, Immunol Unit, Jeddah 21589, Saudi Arabia

[13] Mendel Univ Brno, Fac Agrisci, Dept Anim Morphol Physiol & Genet, Lab Anim Immunol & Biotechnol, Brno 61300, Czech Republic

[14] Jazan Univ, Coll Nursing & Allied Hlth Sci, Res & Sci Studies Unit, Jazan 45142, Saudi Arabia

[15] Lebanese Amer Univ, Gilbert & Rose Marie Chagoury Sch Med, Beirut 11022, Lebanon

[16] Ajman Univ, Ctr Med & Bioallied Hlth Sci Res, Ajman, U Arab Emirates

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2023年 / 165卷

关键词：

Antiproliferative; Apoptosis; DNA nicking; HepG2; Mitochondrial membrane potential (MMP); Reactive oxygen species (ROS); MESENCHYMAL TRANSITION; CANCER; ANTIOXIDANT; PROLIFERATION; STRESS; PLANTS; CYCLE;

D O I：

10.1016/j.biopha.2023.115236

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase - 3 and - 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.

引用

页数：7

共 50 条

[41] Natural Plant Extract Tubeimoside I Promotes Apoptosis-Mediated Cell Death in Cultured Human Hepatoma (HepG2) Cells
Wang, Yashu
Deng, Linhong
Zhong, Hongzhe
Wang, Yajie
Jiang, Xuemei
Chen, Jun
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2011, 34 (06) : 831 - 838
[42] Fuzheng Qingjie recipe induces apoptosis in HepG2 cells via P38 MAPK activation and the mitochondria-dependent apoptotic pathway
Chen, Xu-Zheng
Li, Jin-Nong
Zhang, You-Quan
Cao, Zhi-Yun
Liu, Zhi-Zhen
Wang, Su-Qing
Liao, Lian-Ming
Du, Jian
MOLECULAR MEDICINE REPORTS, 2014, 9 (06) : 2381 - 2387
[43] Leachianone A as a potential anti-cancer drug by induction of apoptosis in human hepatoma HepG2 cells
Cheung, Crystal Sao-Fong
Chung, Karen Ka-Wing
Lui, Julian Chun-Kin
Lau, Ching-Po
Hon, Po-Ming
Chan, Judy Yuet-Wa
Fung, Kwok-Pul
Au, Shannon Wing-Ngor
CANCER LETTERS, 2007, 253 (02) : 224 - 235
[44] A ginseng saponin metabolite-induced apoptosis in HepG2 cells involves a mitochondria-mediated pathway and its downstream caspase-8 activation and Bid cleavage
Oh, SH
Lee, BH
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2004, 194 (03) : 221 - 229
[45] Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma HepG2 cells
Liu, J
Shen, HM
Ong, CN
CANCER LETTERS, 2000, 153 (1-2) : 85 - 93
[46] PATHWAYS FOR THE MUTAGENESIS OF 1-NITROPYRENE AND DINITROPYRENES IN THE HUMAN HEPATOMA-CELL LINE HEPG2
SILVERS, KJ
EDDY, EP
MCCOY, EC
ROSENKRANZ, HS
HOWARD, PC
ENVIRONMENTAL HEALTH PERSPECTIVES, 1994, 102 : 195 - 200
[47] Intracellular glutathione is a cofactor in methylseleninic acid-induced apoptotic cell death of human hepatoma HEPG2 cells
Shen, HM
Ding, WX
Ong, CN
FREE RADICAL BIOLOGY AND MEDICINE, 2002, 33 (04) : 552 - 561
[48] Reactive oxygen species and mitochondria mediate the induction of apoptosis in human hepatoma HepG2 cells by the rodent peroxisome proliferator and hepatocarcinogen, perfluorooctanoic acid
Panaretakis, T
Shabalina, IG
Grandér, D
Shoshan, MC
DePierre, JW
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2001, 173 (01) : 56 - 64
[49] Phenylarsine oxide (PAO) induces apoptosis in HepG2 cells via ROS-mediated mitochondria and ER-stress dependent signaling pathways
Huang, Ping
Zhang, Yu Hua
Zheng, Xiao Wei
Liu, Yu Jia
Zhang, Hong
Fang, Luo
Zhang, Yi Wen
Yang, Chang
Islam, Khairul
Wang, Chao
Naranmandura, Hua
METALLOMICS, 2017, 9 (12) : 1756 - 1764
[50] Hesperidin from Citrus seed induces human hepatocellular carcinoma HepG2 cell apoptosis via both mitochondrial and death receptor pathways
Banjerdpongchai, Ratana
Wudtiwai, Benjawan
Khaw-on, Patompong
Rachakhom, Wasitta
Duangnil, Natthachai
Kongtawelert, Prachya
TUMOR BIOLOGY, 2016, 37 (01) : 227 - 237

← 1 2 3 4 5 →