Apoptosis induction in human hepatoma cell line HepG2 cells by trans-Anethole via activation of mitochondria-mediated apoptotic pathways

被引：6

作者：

Harakeh, Steve ^{[1
,2
]}

Al-Raddadi, Rajaa ^{[3
]}

Alamri, Turki ^{[4
]}

Al-Jaouni, Soad ^{[2
,5
]}

Qari, Mohammed ^{[2
,5
]}

Qari, Yousef ^{[6
]}

Kumar, Ajay ^{[7
]}

Tashkandi, Hanaa M. ^{[8
]}

Moulay, Mohammed ^{[9
,10
]}

Aldahlawi, Alia M. ^{[11
,12
]}

Slama, Petr ^{[13
]}

Haque, Shafiul ^{[14
,15
,16
]}

机构：

[1] King Abdulaziz Univ, King Fahd Med Res Ctr, Jeddah 21589, Saudi Arabia

[2] King Abdulaziz Univ, Fac Med, Yousef Abdul Latif Jameel Sci Chair Prophet Med Ap, Jeddah 21589, Saudi Arabia

[3] King Abdulaziz Univ, Fac Med, Dept Community Med, Jeddah 21589, Saudi Arabia

[4] King Abdulaziz Univ, Fac Med Rabigh, Family & Community Med Dept, Jeddah, Saudi Arabia

[5] King Abdulaziz Univ, King Abdulaziz Univ Hosp, Fac Med, Dept Hematol Pediat Oncol, Jeddah 21589, Saudi Arabia

[6] King Abdulaziz Univ, Fac Med, Dept Med, Jeddah, Saudi Arabia

[7] Chandigarh Univ, Univ Ctr Res & Dev, Biotechnol Engn & Food Technol, Mohali 140413, Punjab, India

[8] King Abdulaziz Univ, Fac Med, Dept Gen Surg, Jeddah 21589, Saudi Arabia

[9] King Abdulaziz Univ, King Fahd Med Res Ctr, Fac Appl Med Sci, Dept Med Lab Sci, Jeddah 21589, Saudi Arabia

[10] King Abdulaziz Univ, Fac Appl Med Sci, Dept Med Lab Sci, Jeddah 21589, Saudi Arabia

[11] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah, Saudi Arabia

[12] King Abdulaziz Univ, King Fahd Med Res Ctr, Immunol Unit, Jeddah 21589, Saudi Arabia

[13] Mendel Univ Brno, Fac Agrisci, Dept Anim Morphol Physiol & Genet, Lab Anim Immunol & Biotechnol, Brno 61300, Czech Republic

[14] Jazan Univ, Coll Nursing & Allied Hlth Sci, Res & Sci Studies Unit, Jazan 45142, Saudi Arabia

[15] Lebanese Amer Univ, Gilbert & Rose Marie Chagoury Sch Med, Beirut 11022, Lebanon

[16] Ajman Univ, Ctr Med & Bioallied Hlth Sci Res, Ajman, U Arab Emirates

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2023年 / 165卷

关键词：

Antiproliferative; Apoptosis; DNA nicking; HepG2; Mitochondrial membrane potential (MMP); Reactive oxygen species (ROS); MESENCHYMAL TRANSITION; CANCER; ANTIOXIDANT; PROLIFERATION; STRESS; PLANTS; CYCLE;

D O I：

10.1016/j.biopha.2023.115236

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase - 3 and - 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.

引用

页数：7

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