Apoptosis induction in human hepatoma cell line HepG2 cells by trans-Anethole via activation of mitochondria-mediated apoptotic pathways

被引：6
|
作者：
Harakeh, Steve [1 ,2 ]
Al-Raddadi, Rajaa [3 ]
Alamri, Turki [4 ]
Al-Jaouni, Soad [2 ,5 ]
Qari, Mohammed [2 ,5 ]
Qari, Yousef [6 ]
Kumar, Ajay [7 ]
Tashkandi, Hanaa M. [8 ]
Moulay, Mohammed [9 ,10 ]
Aldahlawi, Alia M. [11 ,12 ]
Slama, Petr [13 ]
Haque, Shafiul [14 ,15 ,16 ]
机构：
[1] King Abdulaziz Univ, King Fahd Med Res Ctr, Jeddah 21589, Saudi Arabia
[2] King Abdulaziz Univ, Fac Med, Yousef Abdul Latif Jameel Sci Chair Prophet Med Ap, Jeddah 21589, Saudi Arabia
[3] King Abdulaziz Univ, Fac Med, Dept Community Med, Jeddah 21589, Saudi Arabia
[4] King Abdulaziz Univ, Fac Med Rabigh, Family & Community Med Dept, Jeddah, Saudi Arabia
[5] King Abdulaziz Univ, King Abdulaziz Univ Hosp, Fac Med, Dept Hematol Pediat Oncol, Jeddah 21589, Saudi Arabia
[6] King Abdulaziz Univ, Fac Med, Dept Med, Jeddah, Saudi Arabia
[7] Chandigarh Univ, Univ Ctr Res & Dev, Biotechnol Engn & Food Technol, Mohali 140413, Punjab, India
[8] King Abdulaziz Univ, Fac Med, Dept Gen Surg, Jeddah 21589, Saudi Arabia
[9] King Abdulaziz Univ, King Fahd Med Res Ctr, Fac Appl Med Sci, Dept Med Lab Sci, Jeddah 21589, Saudi Arabia
[10] King Abdulaziz Univ, Fac Appl Med Sci, Dept Med Lab Sci, Jeddah 21589, Saudi Arabia
[11] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah, Saudi Arabia
[12] King Abdulaziz Univ, King Fahd Med Res Ctr, Immunol Unit, Jeddah 21589, Saudi Arabia
[13] Mendel Univ Brno, Fac Agrisci, Dept Anim Morphol Physiol & Genet, Lab Anim Immunol & Biotechnol, Brno 61300, Czech Republic
[14] Jazan Univ, Coll Nursing & Allied Hlth Sci, Res & Sci Studies Unit, Jazan 45142, Saudi Arabia
[15] Lebanese Amer Univ, Gilbert & Rose Marie Chagoury Sch Med, Beirut 11022, Lebanon
[16] Ajman Univ, Ctr Med & Bioallied Hlth Sci Res, Ajman, U Arab Emirates
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2023年 / 165卷
关键词：
Antiproliferative; Apoptosis; DNA nicking; HepG2; Mitochondrial membrane potential (MMP); Reactive oxygen species (ROS); MESENCHYMAL TRANSITION; CANCER; ANTIOXIDANT; PROLIFERATION; STRESS; PLANTS; CYCLE;
D O I：
10.1016/j.biopha.2023.115236
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase - 3 and - 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.
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页数：7
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