Antimicrobial evaluation of Tripleurospermum callosum (Boiss. & Heldr.) E. Hossain extracts using in vitro and in vivo Caenorhabditis elegans model against urinary system pathogens

Tripleurospermum callosum (Boiss. & Heldr.) E. Hossain was recorded in Turkish ethnobotanical data for its use against urinary and respiratory system ailments. Infusion, decoction and 96% ethanol extracts of T. callosum aerial parts were prepared for in vitro antimicrobial activity against urinary system pathogens Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Klebsiella aerogenes ATCC 1348 and Candida albicans ATCC 10231. The non-toxic concentrations of extracts and in vivo antimicrobial assay were performed using C. elegans. The extracts were analysed by Liquid Chromatography Mass Spectrometry (LC-MS/MS) for phytochemical composition. The water extracts were non-toxic at between 5000 and 312 mu g/mL, while 96% ethanol extract at 312 mu g/mL for C. elegans. The infusion extract showed in vivo anti-infective effect 5000-312 mu g/mL against Gram-negative strains. The results indicate a potential role of plant extracts with relatively non-toxic and anti-infective effects against urinary system pathogens.