First Isolation and Genome Sequence Analysis of West Nile Virus in Mosquitoes in Brazil

被引：7

作者：

Neto, Joaquim Pinto Nunes ^{[1
]}

Reis, Lucia Aline Moura ^{[2
]}

Freitas, Maria Nazare Oliveira ^{[1
]}

do Nascimento, Bruna Lais Sena ^{[1
]}

das Chagas, Liliane Leal ^{[1
]}

da Costa, Hernan Hermes Monteiro ^{[3
]}

Rodrigues, Jessica Cecilia Pinheiro ^{[4
]}

Braga, Camila Margalho ^{[2
]}

da Silva, Eliana Vieira Pinto ^{[1
]}

Silva, Sandro Patroca ^{[1
]}

Martins, Livia Caricio ^{[1
]}

机构：

[1] Evandro Chagas Inst, Arbovirol & Hemorrhag Fevers Sect, BR-67030000 Ananindeua, PA, Brazil

[2] State Univ Para, Ctr Biol & Hlth Sci, Grad Program Parasitary Biol Amazon, BR-66095663 Belem, PA, Brazil

[3] Adolfo Lutz Inst, Immunol Ctr, BR-01246000 Sao Paulo, SP, Brazil

[4] Evandro Chagas Inst, Lab Anim Husb & Prod Sect, BR-67030000 Ananindeua, PA, Brazil

来源：

TROPICAL MEDICINE AND INFECTIOUS DISEASE | 2023年 / 8卷 / 04期

关键词：

West Nile virus infection; Culicidae; Culex; disease vectors; UNITED-STATES; SINGLE-CELL; NEW-YORK; SURVEILLANCE; INFECTION; HORSES; TRANSMISSION; ARBOVIRUSES; PERFORMANCE; PHYLOGENIES;

D O I：

10.3390/tropicalmed8040237

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

West Nile virus is a flavivirus transmitted by mosquitoes, mainly of the genus Culex. In Brazil, serological studies have already indicated the circulation of the virus since 2003, with the first human case detected in 2014. The objective of the present paper is to report the first isolation of WNV in a Culex (Melanoconion) mosquito. Arthropods were collected by protected human attraction and CDC light bait, and taxonomically identified and analyzed by viral isolation, complement fixation and genomic sequencing tests. WNV was isolated from samples of Culex (Melanoconion) mosquitoes, and the sequencing analysis demonstrated that the isolated strain belonged to lineage 1a. The finding of the present study presents the first evidence of the isolation and genome sequencing of WNV in arthropods in Brazil.

引用

页数：14

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