Chemical Tools to Study Bacterial Glycans: A Tale from Discovery of Glycoproteins to Disruption of Their Function

被引:6
作者
Barrett, Katharine [1 ]
Dube, Danielle H. [1 ]
机构
[1] Bowdoin Coll, Dept Chem & Biochem, 6600 Coll Stn, Brunswick, ME 04011 USA
基金
美国国家卫生研究院;
关键词
glycan; metabolic labelling; bacteria; bioorthogonal chemistry; glycoprotein; O-LINKED GLYCOSYLATION; SUGAR BUILDING-BLOCKS; FREE CLICK CHEMISTRY; PROTEIN GLYCOSYLATION; PILIN GLYCOSYLATION; CAMPYLOBACTER-JEJUNI; CELL-SURFACES; SIALIC-ACID; GENETIC-CHARACTERIZATION; FLAGELLIN GLYCOSYLATION;
D O I
10.1002/ijch.202200050
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Bacteria coat themselves with a dense array of cell envelope glycans that enhance bacterial fitness and promote survival. Despite the importance of bacterial glycans, their systematic study and perturbation remains challenging. Chemical tools have made important inroads toward understanding and altering bacterial glycans. This review describes how pioneering discoveries from Prof. Carolyn Bertozzi's laboratory inspired our laboratory to develop sugar probes to facilitate the study of bacterial glycans. As described below, we used metabolic glycan labelling to install bioorthogonal reporters into bacterial glycans, ultimately permitting the discovery of a protein glycosylation system, the identification of glycosylation genes, and the development of metabolic glycan inhibitors. Our results have provided an approach to screen bacterial glycans and gain insight into their function, even in the absence of detailed structural information.
引用
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页数:12
相关论文
共 121 条
[1]   Not just for Eukarya anymore: protein glycosylation in Bacteria and Archaea [J].
Abu-Qarn, Mehtap ;
Eichler, Jerry ;
Sharon, Nathan .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2008, 18 (05) :544-550
[2]   Comparative bioinformatics analysis of the mammalian and bacterial glycomes [J].
Adibekian, Alexander ;
Stallforth, Pierre ;
Hecht, Marie-Lyn ;
Werz, Daniel B. ;
Gagneux, Pascal ;
Seeberger, Peter H. .
CHEMICAL SCIENCE, 2011, 2 (02) :337-344
[3]   A comparative study of bioorthogonal reactions with azides [J].
Agard, Nicholas J. ;
Baskin, Jeremy M. ;
Prescher, Jennifer A. ;
Lo, Anderson ;
Bertozzi, Carolyn R. .
ACS CHEMICAL BIOLOGY, 2006, 1 (10) :644-648
[4]   Chemical Approaches To Perturb, Profile, and Perceive Glycans [J].
Agard, Nicholas J. ;
Bertozzi, Carolyn R. .
ACCOUNTS OF CHEMICAL RESEARCH, 2009, 42 (06) :788-797
[5]   A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of blomolecules in living systems [J].
Agard, NJ ;
Prescher, JA ;
Bertozzi, CR .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (46) :15046-15047
[6]  
[Anonymous], 2016, ANGEW CHEM, V128, P9447
[7]   A Critical Examination of Escherichia coli Esterase Activity [J].
Antonczak, Alicja K. ;
Simova, Zuzana ;
Tippmann, Eric M. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (42) :28795-28800
[8]   Role of motility and flagellin glycosylation in the pathogenesis of Pseudomonas aeruginosa burn wound infections [J].
Arora, SK ;
Neely, AN ;
Blair, B ;
Lory, S ;
Ramphal, R .
INFECTION AND IMMUNITY, 2005, 73 (07) :4395-4398
[9]   A genomic island in Pseudomonas aeruginosa carries the determinants of flagellin glycosylation [J].
Arora, SK ;
Bangera, M ;
Lory, S ;
Ramphal, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (16) :9342-9347
[10]   Bioanalytical tools for the discovery of eukaryotic glycoproteins applied to the analysis of bacterial glycoproteins [J].
Balonova, Lucie ;
Hernychova, Lenka ;
Bilkova, Zuzana .
EXPERT REVIEW OF PROTEOMICS, 2009, 6 (01) :75-85