Structural characterization and immunomodulatory activity of an acidic polysaccharide from walnut green husk

被引：12

作者：

Wang, Guoliang ^{[1
,2
]}

Yan, Xinpeng ^{[2
]}

Yang, Xi ^{[2
]}

Feng, Luoluo ^{[2
]}

Pang, Huiyan ^{[3
]}

Zhang, Runguang ^{[2
,4
]}

Zhang, Youlin ^{[2
,4
]}

机构：

[1] Yulin Normal Univ, Coll Chem & Food Sci, 1303,Jiaoyu East Rd, Yulin 537000, Guangxi, Peoples R China

[2] Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Xian 710119, Peoples R China

[3] Yulin Normal Univ, Sch Educ Sci, 1303, Jiaoyu East Rd, Yulin 537000, Guangxi, Peoples R China

[4] 620,West Changan Ave, Xian 710119, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2023年 / 110卷

关键词：

Walnut green husk; Polysaccharide; Structural characterization; Immunomodulatory activity; WATER-SOLUBLE POLYSACCHARIDES; MACROPHAGE ACTIVATION; CORDYCEPS-MILITARIS; OXIDATIVE STRESS; ANTIOXIDANT; OBESITY; PECTIN; DAMAGE;

D O I：

10.1016/j.jff.2023.105877

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Acidic walnut green husk polysaccharide with weight average molecular weight of 5.101 kDa was isolated and designated WGHP-2, which was composed of galacturonic acid (69.47 %), galactose (11.18 %), rhamnose (8.67 %), arabinose (3.96 %), glucose (2.21 %), glucuronic acid (2.28 %), xylose (0.83 %), fucose (0.81 %) and mannose (0.59 %). The main glycosidic linkage types of WGHP-2 were -> 4-alpha-D-Glcp-(1 ->, -> 4-alpha-D-GalpA-(1 ->, t-beta-D-GlcpA-(1 ->, -> 2-beta-D-Rhap-(1 ->, t-alpha-D-Galp-(1 ->, -> 4-alpha-D-Galp-(1 -> and -> 2,4)-alpha-D-Manp(1 ->. Immunostimulating assays showed that WGHP-2 could significantly promote the proliferation of RAW264.7 cells, strengthen the phagocytic activity, and up-regulate the production and release of NO, ROS, TNF-alpha, INF-beta and IL -6. Western-blot analysis indicated that MAPK and PI3K/Akt pathways were involved in the activation of macrophages induced by WGHP-2. Overall, our study suggests that WGHP-2 has the potential to be developed as a functional food with good immunostimulatory activity.

引用

页数：14

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