Antimicrobial activities of polyhexamethylene biguanide against biofilm-producing Prototheca bovis causing bovine mastitis
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作者:
Fidelis, Carlos Eduardo
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Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, BrazilUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, Brazil
Fidelis, Carlos Eduardo
[1
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Leite, Renata de Freitas
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Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, BrazilUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, Brazil
Leite, Renata de Freitas
[1
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Garcia, Breno Luis Nery
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Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, BrazilUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, Brazil
Garcia, Breno Luis Nery
[1
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Goncalves, Juliano Leonel
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Michigan State Univ, Coll Vet Med, Dept Large Anim Clin Sci, E Lansing, MI 48824 USAUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, Brazil
Goncalves, Juliano Leonel
[2
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Good, Liam
[3
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dos Santos, Marcos Veiga
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Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, BrazilUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, Brazil
dos Santos, Marcos Veiga
[1
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机构:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635 Pirassununga, SP, Brazil
[2] Michigan State Univ, Coll Vet Med, Dept Large Anim Clin Sci, E Lansing, MI 48824 USA
[3] Univ London, Royal Vet Coll, London NW1 0TU, England
Prototheca spp. is a frequent cause of bovine mastitis and is highly resistant to commonly used disinfectants. This study aimed to: (1) evaluate the antimicrobial activity of polyhexamethylene biguanide (PHMB) against mastitis-causing Prototheca spp., and (2) evaluate the biofilm production ability of Prototheca spp. A total of 85 Prototheca bovis and 2 Prototheca blaschkeae isolates from bovine mastitis cases were submitted to biofilm production assays and antimicrobial susceptibility tests against PHMB and disinfectants commonly used in dairy herds (chlorhexidine digluconate, povidone-iodine, sodium dichloroisocyanurate, and sodium hypochlorite). The minimal inhibitory concentration (MIC) and minimal algicidal concentration (MAC) were determined by microdilution assays. We observed that PHMB (MIC90: >= 2 mu g/mL and MAC(90): >= 4 mu g/mL) and chlorhexidine gluconate (MIC90 and MAC(90): >= 2 mu g/mL) presented the highest antimicrobial activity against P. bovis isolates, followed by sodium dichloroisocyanurate (MIC90 and MAC(90): >= 1,400 mu g/mL), sodium hypochlorite (MIC90 and MAC(90): >= 2,800 mu g/mL), and povidoneio dine (MIC90 and MAC(90): >= 3,200 mu g/mL). Concerning P. blaschkeae isolates, PHMB (MIC and MAC >= 1 mu g/ mL) and chlorhexidine gluconate (MIC and MAC >= 1 mu g/mL) were the disinfectants that presented the lowest concentration values required to inhibit the isolates. Regarding biofilms formation, 63.5% (n = 54/85) of the P. bovis isolates were classified as strong, 28.3% (n = 24/85) moderate, and 8.2% (n = 7/85) weak biofilm producers. In contrast, the P. blaschkeae isolates were classified as weak and moderate biofilm producers. These findings suggest that PHMB has the potential to be used for teat and milking-equipment disinfection for the prevention of mastitis-causing Prototheca spp. in dairy herds.
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Univ Vet Med Vienna, Inst Milk Hyg, Vienna, AustriaUniv Vet Med Vienna, Inst Milk Hyg, Vienna, Austria
Schabauer, Andrea
Zutz, Christoph
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Univ Vet Med Vienna, Inst Milk Hyg, Vienna, Austria
Bioresources & Technol Tulln, Bioact Microbial Metabolites BiMM, Tulln, Austria
Environm Agcy Austria Umweltbundesamt, Dept Chem & Biocides, Vienna, AustriaUniv Vet Med Vienna, Inst Milk Hyg, Vienna, Austria
Zutz, Christoph
Lung, Barbara
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Vet Hlth Serv Lab, Ried Im Innkreis, AustriaUniv Vet Med Vienna, Inst Milk Hyg, Vienna, Austria
Lung, Barbara
Wagner, Martin
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Univ Vet Med Vienna, Inst Milk Hyg, Vienna, AustriaUniv Vet Med Vienna, Inst Milk Hyg, Vienna, Austria
Wagner, Martin
Rychli, Kathrin
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Univ Vet Med Vienna, Inst Milk Hyg, Vienna, AustriaUniv Vet Med Vienna, Inst Milk Hyg, Vienna, Austria
机构:
Konkuk Univ, Coll Vet Med, Ctr One Hlth, 120 Neungdong ro, Seoul 05029, South KoreaKonkuk Univ, Coll Vet Med, Ctr One Hlth, 120 Neungdong ro, Seoul 05029, South Korea
Kim, Hyeon-Jin
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Youn, Hye-Young
Moon, Jin-San
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Anim & Plant Quarantine Agcy, Bacterial Dis Div, 177 Hyeksin 8 ro, Gimcheon 39660, Gyeonsangbuk do, South KoreaKonkuk Univ, Coll Vet Med, Ctr One Hlth, 120 Neungdong ro, Seoul 05029, South Korea
Moon, Jin-San
Kim, Hyunsook
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Hanyang Univ, Coll Human Ecol, Dept Food & Nutr, 222 Wangsimni ro, Seoul 04763, South KoreaKonkuk Univ, Coll Vet Med, Ctr One Hlth, 120 Neungdong ro, Seoul 05029, South Korea
Kim, Hyunsook
Seo, Kun -Ho
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Konkuk Univ, Coll Vet Med, Ctr One Hlth, 120 Neungdong ro, Seoul 05029, South KoreaKonkuk Univ, Coll Vet Med, Ctr One Hlth, 120 Neungdong ro, Seoul 05029, South Korea