Biohydrogen production through energy efficient surfactant induced microwave pretreatment of macroalgae Ulva reticulata

Macroalgal biomass being rich in carbohydrates, proteins and lipids in their cell wall has been considered as the most efficient organic rich sources for biofuel (biohydrogen) production. In this study, Pluronic P-123-induced microwave pretreatment was applied to disintegrate the marine macroalgae biomass, Ulva reticulata. Microwave disintegration was done by varying the treatment time and microwave power from 0 to 40 min and 0.09 KW to 0.63 KW. A maximum chemical oxygen demand (COD) solubilization of 22.33% was achieved at a microwave power and time duration of 0.36 kW and 15 min. Chemical (Pluronic P-123, a mild surfactant) was combined with optimum microwave disintegration conditions to increase the solubilization efficiency and this combined pretreatment achieved a maximum COD solubilization of 31.02% at 10 min pretreatment time and 0.06 g per g TS of Pluronic P-123 dosage. The present study indicated that combination of surfactant with microwave pre-treatment substantially improves the COD solubilization with reduced pretreatment-time than mono microwave pretreatment. An optimal hydrogen yield of 98.37 mL was achieved through this combined pretreatment. The biohydrogen data was modelled with Gompertz model and the kinetic parameters derived through this model implies that the calculated adjusted R squared values for all the samples lies between 0.95 and 0.99. This shows that the model fitted biohydrogen experimental values accurately. In addition, Pluronic P-123-induced micro-wave pretreatment was regarded as energy efficient and cost effective than microwave pretreatment alone with net energy production and a greater energy ratio of 504.38 kWh/Ton macroalgae and 1.2 when compared to microwave pretreatment alone (-2975.6 kWh/Ton macroalgae and 0.5).