A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment

被引：6

作者：

Zhang, Linwei ^{[1
,2
]}

Yin, Xiaoyang ^{[1
,2
]}

Yang, Hongfei ^{[1
,2
]}

Wen, Hao ^{[1
,2
]}

Han, Shiyun ^{[1
,2
]}

Pan, Xiaoming ^{[1
,2
]}

Li, Huaming ^{[1
,2
]}

Peng, Dapeng ^{[1
,2
,3
,4
,5
]}

机构：

[1] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, MOA Key Lab Detect Vet Drug Residues, Wuhan 430070, Peoples R China

[3] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

[4] Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Wuhan 430070, Peoples R China

[5] Chinese Acad Agr Sci, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr,Agr Genom Inst Shenzhen, Shenzhen 518000, Peoples R China

来源：

FOODS | 2024年 / 13卷 / 01期

基金：

中国国家自然科学基金;

关键词：

residue detection; pretilachlor; monoclonal antibody; ic-ELISA; METABOLISM; HERBICIDES; FENCLORIM; WATER;

D O I：

10.3390/foods13010012

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Pretilachlor is a chloroacetamide herbicide mainly used for weed and broadleaf weed control in rice, that is widely utilized in China. In order to detect the residue of pretilachlor in the environment and food, a highly sensitive and specific monoclonal antibody (mAb) against pretilachlor was prepared, and the half maximum inhibitory concentration (IC50) of the monoclonal antibody was validated to be 31.47 +/- 2.35 mu g/L. An indirect competitive ELISA (ic-ELISA) based on the antibody with a linear range of 6.25 similar to 100 mu g/L was developed. The specificity of the antibody was explained by computer simulations and experimental validation. The mAb exhibited negligible cross-reactivity towards alachlor, acetochlor, propisochlor, butachlor, and metalaxyl, and the limits of detection (LOD) for pretilachlor in lake, rice, and soil samples were 4.83 similar to 5.23 mu g/L. The recoveries of all samples were 78.3 similar to 91.3%. The reliability of the ic-ELISA method for residue detection of pretilachlor in the environment and grains was confirmed using high performance liquid chromatography.

引用

页数：13

共 25 条

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