An improved rapid method for DNA recovery from cotton swabs

被引：4
|
作者：
Gray, Alexander [1 ]
Kuffel, Agnieszka [1 ]
Daeid, Niamh Nic [1 ]
机构：
[1] Univ Dundee, Leverhulme Res Ctr Forens Sci, Dundee, Scotland
来源：
FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2023年 / 64卷
关键词：
DNA recovery; Extraction; Cotton swabs; Direct PCR;
D O I：
10.1016/j.fsigen.2023.102848
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We present a novel rapid method for the recovery of cellular and free DNA from cotton swabs based on a simple elution buffer containing a high molecular weight polymer and detergent combined with a short proteinase K digestion to release cellular DNA. This method shows increased yields approaching 80% recovery of the input DNA compared to the QIAamp DNA Mini kit standard extraction protocol for swabs which has a recovery of 20-30%. The buffer components in the described method are compatible with direct PCR analysis of the isolated DNA without further purification. Recovery efficiencies were estimated by qPCR.
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收藏
页数：11
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