Extracting spacing-derived estimates of rod density in healthy retinae

被引：7

作者：

Heitkotter, Heather ^{[1
]}

Patterson, Emily J. ^{[2
]}

Woertz, Erica N. ^{[3
]}

Cava, Jenna A. ^{[4
]}

Gaffney, Mina ^{[5
,6
]}

Adhan, Iniya ^{[7
]}

Tam, Johnny ^{[8
]}

Cooper, Robert F. ^{[4
,5
,6
]}

Carroll, Joseph ^{[1
,4
,5
,6
]}

机构：

[1] Med Coll Wisconsin, Dept Cell Biol Neurobiol & Anat, Milwaukee, WI 53226 USA

[2] UCL, UCL Inst Ophthalmol, London, England

[3] Univ Utah, John Moran Eye Ctr, Salt Lake City, UT USA

[4] Med Coll Wisconsin, Dept Ophthalmol & Visual Sci, Milwaukee, WI 53226 USA

[5] Marquette Univ, Joint Dept Biomed Engn, Milwaukee, WI 53233 USA

[6] Med Coll Wisconsin, Milwaukee, WI 53226 USA

[7] Med Coll Wisconsin, Sch Med, Milwaukee, WI USA

[8] NEI, Natl Inst Hlth, Bethesda, MD USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2023年 / 14卷 / 01期

基金：

美国国家卫生研究院;

关键词：

ASSESSING PHOTORECEPTOR STRUCTURE; PARAFOVEAL CONE DENSITY; ADAPTIVE-OPTICS; AUTOMATIC DETECTION; PACKING DENSITY; SEGMENTATION; ECCENTRICITY; RELIABILITY; STATISTICS; AGREEMENT;

D O I：

10.1364/BOE.473101

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantification of the rod photoreceptor mosaic using adaptive optics scanning light ophthalmoscopy (AOSLO) remains challenging. Here we demonstrate a method for deriving estimates of rod density and rod:cone ratio based on measures of rod spacing, cone numerosity, and cone inner segment area. Twenty-two AOSLO images with complete rod visualization were used to validate this spacing-derived method for estimating density. The method was then used to estimate rod metrics in an additional 105 images without complete rod visualization. The spacing-derived rod mosaic metrics were comparable to published data from histology. This method could be leveraged to develop large normative databases of rod mosaic metrics, though limitations persist with intergrader variability in assessing cone area and numerosity. (c) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement

引用

页码：1 / 17

页数：17