Identification and quantification of DNA N6-methyladenine modification in mammals: A challenge to modern analytical technologies

被引：4
作者：
Lyu, Cong [1 ]
Wang, Hui-Dong [1 ]
Lai, Weiyi [1 ]
Wang, Hailin [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxi col, Beijing 100085, Peoples R China
[2] UCAS, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310024, Peoples R China
[3] Univ Chinese Acad Sci, Beijing, Peoples R China
来源：
CURRENT OPINION IN CHEMICAL BIOLOGY | 2023年 / 73卷
基金：
中国国家自然科学基金;
关键词：
ADENINE METHYLATION; N-6-METHYLADENINE; N-6-ADENINE; BASE;
D O I：
10.1016/j.cbpa.2022.102259
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA N6-methyladenine modification (6mA) is a predominant epigenetic mark in prokaryotes but rarely present in multicel-lular metazoa. The analytical technologies have been devel-oped for sensitive detection of 6mA, including ultra-high performance liquid chromatography coupled with mass spec-trometry (UHPLC-MS/MS) and single molecule real-time sequencing (SMRTseq). However, it remains challenging to detect 6mA at global level and/or in the context of sequence in multicellular metazoa (including mammals). This mini-review brings insights into current dilemma and potential solutions for the identification and quantifications of 6mA in mammals.
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页数：7
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