A supersensitive electrochemical sensor based on RCA amplification-assisted "silver chain"-linked gold interdigital electrodes and CRISPR/Cas9 for the detection of Staphylococcus aureus in food

被引：26

作者：

Zhen, Deshuai ^{[1
,2
,3
]}

Zhang, Shaoqi ^{[1
,2
]}

Yang, Aofeng ^{[2
]}

Ma, Qian ^{[2
]}

Deng, Zhongliang ^{[1
,2
]}

Fang, Jing ^{[2
]}

Cai, Qingyun ^{[3
]}

He, Jun ^{[1
]}

机构：

[1] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China

[2] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China

[3] Hunan Univ, Sch Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China

来源：

FOOD CHEMISTRY | 2024年 / 440卷

基金：

中国国家自然科学基金;

关键词：

S; aureus; CRISPR/Cas9; Rolling circle amplification; Silver chain;

D O I：

10.1016/j.foodchem.2023.138197

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

With the rising emphasis on food safety, technology to rapidly identify Staphylococcus aureus (S. aureus) is of great significance. Herein, we developed a novel electrochemical biosensor based on the CRISPR/Cas9 system and rolling circle amplification (RCA)-assisted "silver chain"-linked gold interdigital electrodes (Au-IDE). This sensor utilizes RCA to create DNA long chains that span the Au-IDE, and CRISPR/Cas9 as a recognition component to recognize capture/target dsDNA. Additionally, we used silver staining technology to improve detection sensitivity. Then, we detected S. aureus through impedance changes that occurred when the silver chain between the Au-IDE was connected or broke, with a limit of detection (LOD) of 7 CFU/mL and a detection time of 1.5 h. Lastly, we successfully employed this sensor to detect S. aureus in real food samples, making it a promising tool for food monitoring.

引用

页数：8

共 37 条

[1] Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies [J].