Development of a Microfluidic Platform for High-Throughput Characterization of Multiple Biophysical Properties of Single-Cell Membranes

被引：0
|
作者：
Yu, Hongfei [1 ]
Chen, Xiao [2 ,3 ]
Zhang, Yi [4 ]
Yang, Shuhao [1 ,5 ]
Gui, Lili [1 ,5 ]
Chen, Deyong [2 ,3 ]
Wang, Junbo [2 ,3 ]
Chen, Jian [2 ,3 ]
Wang, Ke [1 ]
机构：
[1] Beijing Univ Posts & Telecommun, Sch Elect Engn, Beijing 100876, Peoples R China
[2] Chinese Acad Sci, Aerosp Informat Res Inst, State Key Lab Transducer Technol, Beijing 100190, Peoples R China
[3] Univ Chinese Acad Sci, Sch Elect Elect & Commun Engn, Sch Future Technol, Beijing 100049, Peoples R China
[4] Peking Univ, Sch Integrated Circuits, Beijing 100871, Peoples R China
[5] Beijing Univ Posts & Telecommun, State Key Lab Informat Photon & Opt Commun, Beijing 100876, Peoples R China
来源：
2023 IEEE SENSORS | 2023年
基金：
中国国家自然科学基金;
关键词：
single-cell analysis; microfluidics; Single-cell membrane biophysical properties; membrane potential; membrane tension; specific membrane capacitance; DEFORMABILITY;
D O I：
10.1109/SENSORS56945.2023.10325173
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Single-cell membrane biophysical properties, which including membrane potential, membrane tension, and specific membrane capacitance, can serve as label-free indicators for assessing cell type and state. However, conventional tools limited in their throughput, such as patch clamping and AFM, impose constraints in single-cell membrane biophysical properties detection. The application of microfluidics in single-cell biophysical measurements allows for a convenient and efficient detection with the capability of multi-parameter analysis. In this paper, a microfluidic platform for high-throughput characterization of multiple biophysical properties of single-cell membranes was developed. Using this platform, we successfully measured the membrane relative potential, membrane tension, and specific membrane capacitance of single cells.
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页数：4
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