The Dynamics of Synthesis and Localization of Jumbo Phage RNA Polymerases inside Infected Cells

被引：8

作者：

Antonova, Daria ^{[1
]}

Belousova, Viktoriia V. ^{[1
]}

Zhivkoplias, Erik ^{[1
]}

Sobinina, Mariia ^{[1
]}

Artamonova, Tatyana ^{[1
]}

Vishnyakov, Innokentii E. ^{[2
]}

Kurdyumova, Inna ^{[1
]}

Arseniev, Anatoly ^{[3
]}

Morozova, Natalia ^{[1
]}

Severinov, Konstantin ^{[3
,4
,5
]}

Khodorkovskii, Mikhail ^{[1
]}

Yakunina, Maria V. ^{[1
]}

机构：

[1] Peter Great St Petersburg Polytech Univ, Res Ctr Nanobiotechnol, Peter Great St, St Petersburg 195251, Russia

[2] Russian Acad Sci, Inst Cytol, Grp Mol Cytol Prokaryotes & Bacterial Invas, St Petersburg 194064, Russia

[3] Russian Acad Sci, Inst Gene Biol, Ctr Precis Genome Editing & Genet Technol Biomed, Moscow 119334, Russia

[4] Natl Kurchatov Ctr, Inst Mol Genet, Moscow 123182, Russia

[5] State Univ New Jersey, Waksman Inst Microbiol, Rutgers, Piscataway, NJ 08854 USA

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 10期

基金：

俄罗斯科学基金会;

关键词：

jumbo phages; phiKZ; transcription regulation; phage nucleus; virion RNA-polymerase; giant phages; pseudo-nucleus; BACTERIOPHAGE PHI-KZ; NUCLEUS; DNA; GENOME; AR9;

D O I：

10.3390/v15102096

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A nucleus-like structure composed of phage-encoded proteins and containing replicating viral DNA is formed in Pseudomonas aeruginosa cells infected by jumbo bacteriophage phiKZ. The PhiKZ genes are transcribed independently from host RNA polymerase (RNAP) by two RNAPs encoded by the phage. The virion RNAP (vRNAP) transcribes early viral genes and must be injected into the cell with phage DNA. The non-virion RNAP (nvRNAP) is composed of early gene products and transcribes late viral genes. In this work, the dynamics of phage RNAPs localization during phage phiKZ infection were studied. We provide direct evidence of PhiKZ vRNAP injection in infected cells and show that it is excluded from the phage nucleus. The nvRNAP is synthesized shortly after the onset of infection and localizes in the nucleus. We propose that spatial separation of two phage RNAPs allows coordinated expression of phage genes belonging to different temporal classes.

引用

页数：10

共 37 条

[11]

FALCO SC, 1980, J BIOL CHEM, V255, P4339

[12] Structure of the bacteriophage PhiKZ non-virion RNA polymerase [J].