Effect of laser energy on protein conformation and lipid structure in skin tissue

This study explored the collagen distribution behavior, protein and lipid structure and function changes in the three-layer microstructure of skin tissue in the process of skin welding under different laser linear energies by Raman spectroscopy. The collagen deposition is a reversible thermal damage caused by laser linear energy not more than 7 J/m. Linear energy of 10 J/m can effectively increase the functional expression of lipoprotein, tryptophan and ceramide in the dermis, and can control the vibration of peptide chain in the reversible range. Also, the functions of various typical amino acids in the cuticle and epidermis can be well expressed under the condition of 7 J/m linear energy, while the damage of polypeptide chains is serious when the energy exceeds 12 J/m. The thermal stability of lipid components was significantly higher than that of collagen. The Raman peak intensity of sample responses were similar under the linearar energy conditions of 10 J/m and 5 J/m. At the same laser power, the higher the laser scanning speed, the smaller the thermal damage and the similar lipid transient structure could be obtained.