Hypoglycemic effect of recrystallized resistant starch on high-fat diet- and streptozotocin-induced type 2 diabetic mice via gut microbiota modulation

The hypoglycemic effects of two recrystallized resistant starches, A -type (ARS) and B -type (BRS), were investigated in type 2 diabetic mice. Mice were treated with low-, medium-, or high -dose ARS, high -dose BRS, or highdose ARS combined with BRS (ABRS). After 10 weeks of continuous intervention, the medium -dose ARS group showed a significant reduction in fasting blood glucose, area under the curve of glucose, triglyceride (P < 0.01), and low -density lipoprotein (P < 0.05) levels compared to the model group and an increase in high -density lipoprotein levels (P < 0.01). The peptide YY and glucagon-like peptide -1 levels in the high -dose ARS, BRS, and ABRS groups and the butyric acid yield in the medium -dose ARS and BRS groups were significantly increased (P < 0.01) compared to those in the model group. Medium- and high -dose ARS intervention efficiently increased the relative abundance of beneficial Bacteroidetes, Lactobacillus, Lachnospiraceae_NK4A136_group, and Faecalibaculum, and lowered the ratio of Firmicutes to Bacteroidetes. Overall, ARS exhibited greater advantages than BRS in lowering blood sugar levels.