Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes

被引：1

作者：

Zheng, Ying ^{[1
,2
]}

Xiao, Xia ^{[1
,2
]}

Li, Zhenzhen ^{[1
,2
]}

Shao, Yong ^{[1
,2
]}

Chen, Jieyi ^{[1
,2
]}

Guo, Zhouyi ^{[1
,2
]}

Zhong, Huiqing ^{[1
,2
]}

Liu, Zhiming ^{[1
,2
]}

机构：

[1] South China Normal Univ, MOE Key Lab Laser Life Sci, Guangzhou, Peoples R China

[2] South China Normal Univ, Coll Biophoton, Guangdong Prov Key Lab Life Sci, Guangzhou, Peoples R China

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2023年 / 196期

基金：

中国国家自然科学基金;

关键词：

SERS; NANOPARTICLES; MEMBRANE; CELLS;

D O I：

10.3791/65524

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Surface-enhanced Raman scattering (SERS) technology has attracted more and more attention in the biomedical field due to its ability to provide molecular fingerprint information of biological samples, as well as its potential in single-cell analysis. This work aims to establish a simple strategy for label-free SERS bioanalysis based on Au@carbon dot nanoprobes (Au@CDs). Here, polyphenol-derived CDs are utilized as the reductant to rapidly synthesize core-shell Au@CD nanostructures, which allows powerful SERS performance even when the concentration of methylene blue (MB) is as low as 10-9 M, due to the cooperative Raman enhancement mechanism. For bioanalysis, Au@CDs can serve as a unique SERS nanosensor to identify the cellular components of biosamples (e.g., cancer cells and bacteria). The molecular fingerprints from different species can be further distinguished after combination with the principal component analysis. In addition, Au@CDs also enable label-free SERS imaging to analyze intracellular composition profiles. This strategy offers a feasible, label-free SERS bioanalysis, opening up a new prospect for nanodiagnosis.

引用

页数：13

共 40 条

[1] Surface-Enhanced Raman Scattering Spectroscopy for Label-Free Analysis of P. aeruginosa Quorum Sensing [J].