The crystal structure of the human smacovirus 1 Rep domain

被引：1

作者：

Limon, Lidia K. ^{[1
]}

Shi, Ke ^{[1
]}

Dao, Amy ^{[1
]}

Rugloski, Jacob ^{[1
]}

Tompkins, Kassidy J. ^{[1
]}

Aihara, Hideki ^{[1
]}

Gordon, Wendy R. ^{[1
]}

Evans, Robert L., III ^{[1
]}

机构：

[1] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2023年 / 79卷

基金：

美国国家卫生研究院;

关键词：

HUH endonucleases; Rep domains; HUH-tags; ssDNA; bioconjugation; smacoviruses; crystal structure; SINGLE-STRANDED-DNA; NUCLEASE DOMAIN; PROTEIN; INITIATION; SEQUENCE; BINDING; SPECIFICITY; CLEAVAGE; MOTIFS;

D O I：

10.1107/S2053230X23009536

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 angstrom resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.

引用

页码：295 / 300

页数：6

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