Dextrose, maltose and starch guide crystallization of strontium-substituted hydroxyapatite: A comparative study for bone tissue engineering application

The influence of carbohydrates on the crystallization of metal-substituted hydroxyapatite predicts its relevance to natural bone growth. This study demonstrates the role of carbohydrates in the crystallization of strontium-substituted hydroxyapatite (SHAP). The increasing order of hydroxyl groups, dextrose (monosaccharide) < maltose (disaccharide) < starch (polysaccharide), coordinated with Ca2+/Sr2+ and thus guided SHAP crystalli-zation, with crystal size reduced from 35 to 19 nm, lattice volume increased from 518 to 537 & ANGS;3, and residual carbohydrates increased from 1.8 to 20.2 %. The variation in residual carbohydrates is due to their interaction with apatite and/or aqueous insolubility. Compared to pure SHAP, the starch-SHAP with higher residual starch showed increased water uptake from 1.23 & PLUSMN; 0.18 to 4.26 & PLUSMN; 0.21 % and degradation from 0.22 & PLUSMN; 0.06 to 1.53 & PLUSMN; 0.14 %, but decreased microhardness from 0.73 & PLUSMN; 0.12 to 0.38 & PLUSMN; 0.01 GPa and protein affinity from 4.82 & PLUSMN; 0.01 to 0.81 & PLUSMN; 0.01 & mu;g/mg. However, its microhardness value was bone-like, and the reduced protein adsorption was masked by the rich osteogenic behaviour. In vitro cellular response demonstrated that the residual carbohydrate and strontium augmented osteocompatibility, proliferation, differentiation and biomineralization. The result concludes that carbohydrates drive SHAP crystallization, and starch-SHAP replicates natural bone.