Spatial quantification of single cell mRNA and ligand binding of the kappa opioid receptor in the rat hypothalamus

被引：4
作者：
Krogsbaek, Maiken [1 ,7 ]
Larsen, Nick Yin [1 ,2 ]
Landau, Anne M. [3 ,4 ]
Sanchez, Connie [4 ,5 ]
Nyengaard, Jens Randel [1 ,6 ]
机构：
[1] Aarhus Univ, Core Ctr Mol Morphol, Dept Clin Med, Aarhus, Denmark
[2] Aarhus Univ, Ctr Functionally Integrat Neurosci, Dept Clin Med, Aarhus, Denmark
[3] Aarhus Univ Hosp, PET Ctr, Dept Nucl Med, Aarhus, Denmark
[4] Aarhus Univ, Dept Clin Med, Translat Neuropsychiat Unit, Aarhus, Denmark
[5] Alkermes Inc, Waltham, MA USA
[6] Aarhus Univ Hosp, Dept Pathol, Aarhus, Denmark
[7] Aarhus Univ Hosp, Palle Juul Jensens Blvd 99, Level 1, C112-C113, DK-8200 Aarhus, Denmark
来源：
JOURNAL OF CHEMICAL NEUROANATOMY | 2023年 / 127卷
关键词：
Spatial image quantification; RNAscope; Autoradiography; Kappa Opioid Receptor; Hypothalamus; ESTROUS-CYCLE; MU; DELTA; EXPRESSION; FOREBRAIN; NEURONS; BRAIN; SEX; CNS;
D O I：
10.1016/j.jchemneu.2022.102205
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Detailed quantification of brain tissue provides a deeper understanding of changes in expression and function. We have created a pipeline to study the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus using high resolution fluorescence microscopy and receptor autoradiography. The workflow involved structured serial sampling of rat hypothalamic nuclei, in situ detection of mRNA and receptor expres-sion, and advanced image analysis. Our results demonstrate how maintaining spatial information can lead to increased understanding of RNA and protein expression. In addition, we show the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus.
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页数：9
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