Metabolic Engineering of Yarrowia lipolytica for Zeaxanthin Production

被引：13
作者：
Zhang, Guilin [1 ,2 ]
Chen, Jing [1 ,2 ]
Wang, Yongzhen [1 ,2 ]
Liu, Zhen [1 ,2 ]
Mao, Xiangzhao [1 ,2 ,3 ]
机构：
[1] Ocean Univ China, Coll Food Sci & Engn, Qingdao Key Lab Food Biotechnol, Qingdao 266404, Peoples R China
[2] China Natl Light Ind, Key Lab Biol Proc Aquat Prod, Qingdao 266404, Peoples R China
[3] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 37期
基金：
中国国家自然科学基金;
关键词：
zeaxanthin; Yarrowia lipolytica; beta-carotene; metabolic engineering; modularenzyme assembly; BIOTECHNOLOGICAL PRODUCTION; SUBCHRONIC TOXICITY;
D O I：
10.1021/acs.jafc.3c01772
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Zeaxanthin is a carotenoid, a dihydroxy derivative of ss-carotene. Zeaxanthin has antioxidant, anti-inflammatory, anticancer, and neuroprotective properties. In this study, Yarrowia lipolytica was used as a host for the efficient production of zeaxanthin. The strain Y. lipolytica PO1h was used to construct the following engineered strains for carotenoid production since it produced the highest ss-carotene among the Y. lipolytica PO1h- and Y. lipolytica PEX17-HA-derived strains. By regulating the key nodes on the carotenoid pathway through wild and mutant enzyme comparison and successive modular assembly, the ss-carotene concentration was improved from 19.9 to 422.0 mg/L. To provide more precursor mevalonate, heterologous genes mvaE and mvaSMT were introduced to increase the production of ss-carotene by 27.2% to the yield of 536.8 mg/L. The ss-carotene hydroxylase gene crtZ was then transferred, resulting in a yield of zeaxanthin of 326.5 mg/L. The oxidoreductase RFNR1 and CrtZ were then used to further enhance zeaxanthin production, and the yield of zeaxanthin was up to 775.3 mg/L in YPD shake flask.
引用
收藏
页码：13828 / 13837
页数：10
相关论文
共 37 条
[1]   Lutein and zeaxanthin reduce A2E and iso-A2E levels and improve visual performance in Abca4-/-/Bco2-/- double knockout mice [J].
Arunkumar, Ranganathan ;
Gorusupudi, Aruna ;
Li, Binxing ;
Blount, J. David ;
Nwagbo, Uzoamaka ;
Kim, Hye Jin ;
Sparrow, Janet R. ;
Bernstein, Paul S. .
EXPERIMENTAL EYE RESEARCH, 2021, 209
[2]   Sources, health benefits, and biological properties of zeaxanthin [J].
Bouyahya, Abdelhakim ;
El Omari, Nasreddine ;
Hakkur, Meryem ;
El Hachlafi, Naoufal ;
Charfi, Saoulajan ;
Balahbib, Abdelaali ;
Guaouguaou, Fatima-Ezzahrae ;
Rebezov, Maksim ;
Maksimiuk, Nikolai ;
Shariati, Mohammad Ali ;
Zengin, Gokhan ;
El Menyiy, Naoual ;
Chamkhi, Imane ;
Bakrim, Saad .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2021, 118 :519-538
[3]   Genetic modification of the carotenoid pathway in the red yeast Xanthophyllomyces dendrorhous: Engineering of a high-yield zeaxanthin strain [J].
Breitenbach, Juergen ;
Pollmann, Hendrik ;
Sandmann, Gerhard .
JOURNAL OF BIOTECHNOLOGY, 2019, 289 :112-117
[4]   Heterologous production of the epoxycarotenoid violaxanthin in Saccharomyces cerevisiae [J].
Cataldo, Vicente F. ;
Arenas, Natalia ;
Salgado, Valeria ;
Camilo, Conrado ;
Ibanez, Francisco ;
Agosin, Eduardo .
METABOLIC ENGINEERING, 2020, 59 :53-63
[5]   Chemical vs. biotechnological synthesis of C13-apocarotenoids: current methods, applications and perspectives [J].
Cataldo, Vicente F. ;
Lopez, Javiera ;
Carcamo, Martin ;
Agosin, Eduardo .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (13) :5703-5718
[6]   Zeaxanthin exerts protective effects on acetic acid-induced colitis in rats via modulation of pro-inflammatory cytokines and oxidative stress [J].
El-Akabawy, Gehan ;
El-Sherif, Neveen M. .
BIOMEDICINE & PHARMACOTHERAPY, 2019, 111 :841-851
[7]  
El-Baz Farouk K, 2021, Toxicol Rep, V8, P1899, DOI [10.1016/j.toxrep.2021.11.021, 10.1016/j.toxrep.2021.11.021]
[8]   A novel gastroprotective effect of zeaxanthin against stress-induced gastritis in male rats targeting the expression of HIF-1α, TFF-1 and MMP-9 through PI3K/Akt/JNK signaling pathway [J].
Elhadidy, Mona G. ;
El Nashar, Eman Mohamad ;
Alghamdi, Mansour A. ;
Samir, Shereen M. .
LIFE SCIENCES, 2021, 273
[9]   Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous β-carotene production [J].
Gao, Shuliang ;
Tong, Yangyang ;
Zhu, Li ;
Ge, Mei ;
Zhang, Yian ;
Chen, Daijie ;
Jiang, Yu ;
Yang, Sheng .
METABOLIC ENGINEERING, 2017, 41 :192-201
[10]   Multiple iso-proteins of FNR in Arabidopsis:: evidence for different contributions to chloroplast function and nitrogen assimilation [J].
Hanke, GT ;
Okutani, S ;
Satomi, Y ;
Takao, T ;
Suzuki, A ;
Hase, T .
PLANT CELL AND ENVIRONMENT, 2005, 28 (09) :1146-1157
1234