Line-field confocal optical coherence tomography for in vivo visualization of morphological characteristics of squamous cell carcinoma in a murine model

被引：1
作者：
Olesen, Uffe H. [1 ,4 ]
Foged, Camilla [1 ]
Jacobsen, Kevin [1 ]
Ortner, Vinzent K. [1 ]
Fredman, Gabriella [1 ]
Paasch, Uwe [2 ]
Haedersdal, Merete [1 ,3 ]
机构：
[1] Univ Copenhagen, Dept Dermatol, Hosp Bispebjerg, Copenhagen, Denmark
[2] Univ Leipzig, Dept Dermatol Venereol & Allergy, Leipzig, Germany
[3] Univ Copenhagen, Fac Hlth & Med Sci, Dept Clin Med, Copenhagen, Denmark
[4] Univ Copenhagen, Dept Dermatol & Venerol, Hosp Bispebjerg, Nielsine Nielsen Vej 9, DK-2400 Copenhagen NV, Denmark
来源：
LASERS IN SURGERY AND MEDICINE | 2024年 / 56卷 / 01期
关键词：
image features; non-invasive imaging; preclinical models; squamous cell carcinoma morphology; ACTINIC KERATOSIS; SKIN-CANCER;
D O I：
10.1002/lsm.23750
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
ObjectivesNon-invasive imaging with line-field confocal optical coherence tomography (LC-OCT) can support the diagnosis of squamous cell carcinoma (SCC) through visualization of morphological characteristics specific to skin cancer. We aimed to visualize prominent morphological characteristics of SCC using LC-OCT in a well-established murine SCC model.Materials and MethodsNine hairless mice were exposed to ultraviolet radiation three times weekly for 9 months to induce SCC development. Visible SCC tumors (n = 9) were imaged with LC-OCT and the presence of 10 well-described morphological characteristics of SCC were evaluated in the scans by two physicians with adjudication by a third.ResultsOverall, murine morphological characteristics resembled corresponding features previously reported in human SCCs. Interrupted dermal-epidermal junction occurred in 100% of tumors. In epidermis, the most frequently observed characteristics were severe epidermal dysplasia (100%) and tumor budding (89%). Common dermal characteristics included broad strands (100%) and collagen alterations (78%).ConclusionLC-OCT imaging can be used to non-invasively visualize morphological characteristics specific to SCC in an in vivo preclinical model.
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页码：14 / 18
页数：5
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