In vitro evaluation of cytotoxic potential of essential oil extracted from leaves of Croton heliotropiifolius Kunth in human tumor cells

被引:5
作者
Cavalcanti, Bruno Coelho [1 ,5 ]
Magalhaes, Islay Lima [1 ]
Rocha, Danilo Damasceno [1 ]
Barreto, Francisco Stefanio [1 ]
de Andrade Neto, Joao Batista [1 ,2 ]
Magalhaes, Hemerson Iury Ferreira [3 ]
dos Santos, Claudio Costa [1 ,4 ]
de Moraes, Manoel Odorico [1 ]
机构
[1] Univ Fed Ceara, Drug Res & Dev Ctr, Fortaleza, Brazil
[2] Christus Univ Ctr UNICHRISTUS, Fortaleza, Brazil
[3] Univ Fed Paraiba, Dept Pharmaceut Sci, Joao Pessoa, Brazil
[4] Fed Univ Semiarid Reg, Dept Engn & Technol, Mossoro, Brazil
[5] Univ Fed Ceara, Drug Res & Dev Ctr, BR-60430160 Fortaleza, CE, Brazil
来源
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | 2024年 / 87卷 / 03期
关键词
Croton heliotropiifolius; cytotoxicity; cancer cells; oxidative stress; DNA damage; ANTIBACTERIAL ACTIVITY; CHEMICAL-CONSTITUENTS; OXIDATIVE STRESS; NATURAL-PRODUCTS; DRUG DISCOVERY; FPG PROTEIN; COMET ASSAY; DNA-DAMAGE; APOPTOSIS; BIODIVERSITY;
D O I
10.1080/15287394.2023.2276894
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Croton heliotropiifolius Kunth, popularly known as "velame," is a shrub that resides in northeastern Brazil. The essential oil of C. heliotropiifolius contains high concentrations of volatile compounds in the leaves and is widely used in folk medicine for many purposes as an antiseptic, analgesic, sedative, and anti-inflammatory agent. Due to the apparent limited amount of information, the aim of this study was to determine the cytotoxic potential of essential oil extracted from leaves of C. heliotropiifolius, utilizing different human cancer cell lines (HL-60, leukemia; HCT-116, colon; MDA-MB435, melanoma; SF295, glioblastoma) and comparison to murine fibroblast L929 cell line. The chemical characterization of the essential oil revealed the presence of large amounts of monoterpenes and sesquiterpenes, the majority of which were aristolene (22.43%), germacrene D (11.38%), Gamma-terpinene (10.85%), and limonene (10.21%). The essential oil exerted significant cytotoxicity on all cancer cells, with low activity on murine L929 fibroblasts, independent of disruption of cell membranes evidenced by absence of hemolytic activity. The cytotoxicity identified was associated with oxidative stress, which culminated in mitochondrial respiration dysfunction and direct or indirect DNA damage (strand breaks and oxidative damage), triggering cell death via apoptosis. Our findings suggest that extracts of essential oil of C. Heliotropiifolius may be considered as agents to be used therapeutically in treatment of certain cancers.
引用
收藏
页码:91 / 107
页数:17
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