Optimization Of the Culture Medium of Althiomycin-Producing Myxococcus stipitatus GL41 Using Plackett-Burman Design and Response Surface Methodology

被引:0
作者
Nguyen, Yen Thi Ngoc [1 ]
Duong, Chung Dinh [1 ]
Nguyen, Thai Minh [2 ]
Nguyen, Nga Dinh [2 ]
Nguyen, Anh Tu [2 ]
机构
[1] Nguyen Tat Thanh Univ, Fac Pharm, Ho Chi Minh City, Vietnam
[2] Univ Med & Pharm Ho Chi Minh City, Fac Pharm, Ho Chi Minh City, Vietnam
来源
JOURNAL OF POPULATION THERAPEUTICS AND CLINICAL PHARMACOLOGY | 2023年 / 30卷 / 06期
关键词
Myxococcus stipitatus; althiomycin; optimization; Plackett-Burman; Response surface methodology; IDENTIFICATION; METABOLISM; XANTHUS;
D O I
10.47750/jptcp.2023.30.06.034
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Althiomycin is a thiazole antibiotic with broad-spectrum activity against Gram-positive and Gram-negative bacteria through protein synthesis inhibition at the site of the 50S ribosomal subunit. This study was conducted to improve althiomycin production in a wild myxobacterial strain (Myxococcus stipitatus GL41) by optimization of the fermentation medium. The Plackett-Burman design screened eleven critical factors that affect althiomycin content in extracts. The factors with significant effect (p < 0.05) were employed in the Box-Behnken model to clarify the interaction between the independent variables and identify the optimal value for each variable. Three factors (soluble starch, baker's yeast and hepes buffer) determined from the Plackett-Burman design regression analysis results showed significant effects on althiomycin production. The RSM model predicted the optimal fermentation medium's parameters, including 6.09 g/L of soluble starch, 4.05 g/L of baker's yeast, and 20.65 g/L of hepes buffer. Model validation was performed to estimate the althiomycin content showed that the value reached 3.3 mg/L, which is 4.7 times higher than the initial P medium. The mathematical model is well compatible with the experimental results showing that optimization of the fermentation medium by statistical algorithms is an effective solution to promote the production yield of natural compounds.
引用
收藏
页码:E287 / E296
页数:10
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