Design of an Electrostatic Frequency Map for the NH Stretch of the Protein Backbone and Application to Chiral Sum Frequency Generation Spectroscopy

被引:10
作者
Konstantinovsky, Daniel [1 ,2 ]
Perets, Ethan A. [1 ,3 ]
Santiago, Ty [1 ]
Olesen, Kristian [1 ]
Wang, Zhijie [1 ]
Soudackov, Alexander V. [1 ]
Yan, Elsa C. Y. [1 ]
Hammes-Schiffer, Sharon [1 ,2 ]
机构
[1] Yale Univ, Dept Chem, New Haven, CT 06520 USA
[2] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[3] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
基金
美国国家卫生研究院;
关键词
AMIDE-I; VIBRATIONAL SPECTROSCOPY; MOLECULAR-ENERGIES; LIQUID WATER; DILUTE HOD; SIDE-CHAIN; PEPTIDE; SPECTRA; INTERFACES; MODES;
D O I
10.1021/acs.jpcb.3c00217
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We develop an electrostatic map for the vibrational NH stretch (amide A) of the protein backbone with a focus on vibrational chiral sum frequency generation spectroscopy (chiral SFG). Chiral SFG has been used to characterize protein secondary structure at interfaces using the NH stretch and to investigate chiral water superstructures around proteins using the OH stretch. Interpretation of spectra has been complicated because the NH stretch and OH stretch overlap spectrally. Although an electrostatic map for water OH developed by Skinner and co-workers was used previously to calculate the chiral SFG response of water structures around proteins, a map for protein NH that is directly responsive to biological complexity has yet to be developed. Here, we develop such a map, linking the local electric field to vibrational frequencies and transition dipoles. We apply the map to two protein systems and achieve much better agreement with experiment than was possible in our previous studies. We show that couplings between NH and OH vibrations are crucial to the line shape, which informs the interpretation of chiral SFG spectra, and that the chiral NH stretch response is sensitive to small differences in structure. This work increases the utility of the NH stretch in biomolecular spectroscopy.
引用
收藏
页码:2418 / 2429
页数:12
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