Hsa_circ_0092856 Promoted the Proliferation, Migration, and Invasion of NSCLC Cells by Up-Regulating the Expression of eIF3a

被引：0
作者：
Yuan, Fuqiang [1 ,2 ]
Huang, Masha [1 ,2 ,3 ]
Huang, Hanxue [1 ,2 ]
Mao, Xiaoyuan [1 ,2 ]
Xie, Pan [1 ,2 ]
Li, Xi [1 ,2 ]
Gao, Yang [4 ]
Zeng, Feiyue [5 ]
Liu, Zhaoqian [1 ,2 ]
机构：
[1] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Dept Clin Pharmacol, Changsha 410008, Peoples R China
[2] Cent South Univ, Inst Clin Pharmacol, Res Ctr Appl Technol Pharmacogen, Minist Educ,Engn, Changsha 410078, Peoples R China
[3] Shanghai Jiao Tong Univ, Coll Basic Med Sci, Sch Med, Dept Biochem & Mol Cell Biol, Shanghai 200025, Peoples R China
[4] Cent South Univ, Xiangya Hosp, Dept Thorac Surg, Changsha 410008, Peoples R China
[5] Cent South Univ, Xiangya Hosp, Dept Radiol, Changsha 410008, Peoples R China
来源：
BIOMEDICINES | 2024年 / 12卷 / 01期
基金：
中国国家自然科学基金;
关键词：
circRNA; hsa_circ_0092856; eIF3a; NSCLC; progression; PLATINUM-BASED CHEMOTHERAPY; LUNG-CANCER; CISPLATIN RESISTANCE; CIRCULAR RNAS; PROGRESSION; CONTRIBUTES; PROGNOSIS; APOPTOSIS;
D O I：
10.3390/biomedicines12010247
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Circular RNA (circRNA) plays a very important regulatory role in a variety of human malignancies such as non-small-cell lung cancer (NSCLC). In the current study, we explored the role of hsa_circ_0092856 in the progression of NSCLC. We screened CircRNA from the eIF3a gene in the Circbase database. The biological functions of hsa_circ_0092856 in NSCLC were analyzed via qRT-PCR, a CCK-8 assay, a plate cloning experiment, scratch testing, a transwell chamber experiment, an RNA nuclear mass separation experiment, an RIP experiment, and a Western blot test. The results showed that hsa_circ_0092856 was highly expressed in NSCLC cells, and the knockdown of hsa_circ_0092856 could inhibit the proliferation, migration, and invasion of NSCLC cells. The overexpression of hsa_circ_0092856 has the opposite effect. The expression of eIF3a also changed with the change in hsa_circ_0092856. These results suggest that hsa_circ_0092856 may play a key role in the progression of NSCLC by regulating the expression of eIF3a.
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页数：13
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