A transcriptional Regulator Gar Regulates the Growth and Virulence of Xanthomonas oryzae pv. oryzae

被引：0
作者：
Wang, Huihui [1 ]
Chen, Fan [1 ]
Tang, Guiyu [1 ]
Ke, Wenli [1 ]
Wang, Shasha [1 ]
Zheng, Dehong [1 ,3 ]
Ruan, Lifang [1 ,2 ]
机构：
[1] Huazhong Agr Univ, Coll Life Sci & Technol, Natl Key Lab Agr Microbiol, Wuhan, Peoples R China
[2] Tibet Agr & Anim Husb Univ, Coll Resources & Environm, Nyingchi, Peoples R China
[3] Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangxi Key Lab Agroenvironm & Agroprod Safety, Nanning, Peoples R China
来源：
CURRENT MICROBIOLOGY | 2023年 / 80卷 / 09期
基金：
中国国家自然科学基金;
关键词：
DIFFUSIBLE SIGNAL FACTOR; CAMPESTRIS; SECRETION; PATHOGENS; ZUR;
D O I：
10.1007/s00284-023-03396-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight, one of the most devastating diseases of rice. Pathogenic bacteria possess numerous transcriptional regulators to participate in the regulation of cellular processes. Here, we identified a transcriptional regulator Gar (PXO_RS11965) that is involved in regulating the growth and virulence of Xoo. Notably, the knockout of gar in Xoo enhanced bacterial virulence to the host rice. RNA-sequencing analysis and quantitative & beta;-glucuronidase (GUS) assay indicated that Gar positively regulates the expression of a & sigma;(54) factor rpoN2. Further experiments confirmed that overexpression of rpoN2 restored the phenotypic changes caused by gar deletion. Our research revealed that Gar influences bacterial growth and virulence by positively regulating the expression of rpoN2.
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页数：11
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共 32 条
[1]   Persistence: mechanisms for triggering and enhancing phenotypic variability [J].
Balaban, N. Q. .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2011, 21 (06) :768-775
[2]   Regulation and secretion of Xanthomonas virulence factors [J].
Buettner, Daniela ;
Bonas, Ulla .
FEMS MICROBIOLOGY REVIEWS, 2010, 34 (02) :107-133
[3]   RETRACTED: The Xanthomonas Type III Effector XopD Targets the Arabidopsis Transcription Factor MYB30 to Suppress Plant Defense (Retracted article. See vol. 30, pg. 253, 2018) [J].
Canonne, Joanne ;
Marino, Daniel ;
Jauneau, Alain ;
Pouzet, Cecile ;
Briere, Christian ;
Roby, Dominique ;
Rivas, Susana .
PLANT CELL, 2011, 23 (09) :3498-3511
[4]   fleQ, the gene encoding the major flagellar regulator of Pseudomonas aeruginosa, is σ70 dependent and is downregulated by Vfr, a homolog of Escherichia coli cyclic AMP receptor protein [J].
Dasgupta, N ;
Ferrell, EP ;
Kanack, KJ ;
West, SEH ;
Ramphal, R .
JOURNAL OF BACTERIOLOGY, 2002, 184 (19) :5240-5250
[5]   Spatial modelling of rice yield losses in Tanzania due to bacterial leaf blight and leaf blast in a changing climate [J].
Duku, Confidence ;
Sparks, Adam H. ;
Zwart, Sander J. .
CLIMATIC CHANGE, 2016, 135 (3-4) :569-583
[6]   A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels [J].
Hickman, JW ;
Tifrea, DF ;
Harwood, CS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (40) :14422-14427
[7]   The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters [J].
Huang, Dong-Liang ;
Tang, Dong-Jie ;
Liao, Qing ;
Li, Heng-Cong ;
Chen, Qi ;
He, Yong-Qiang ;
Feng, Jia-Xun ;
Jiang, Bo-Le ;
Lu, Guang-Tao ;
Chen, Baoshan ;
Tang, Ji-Liang .
NUCLEIC ACIDS RESEARCH, 2008, 36 (13) :4295-4309
[8]   The Zur of Xanthomonas campestris Is Involved in Hypersensitive Response and Positively Regulates the Expression of the hrp Cluster Via hrpX But Not hrpG [J].
Huang, Dong-Liang ;
Tang, Dong-Jie ;
Liao, Qing ;
Li, Xiao-Qian ;
He, Yong-Qiang ;
Feng, Jia-Xun ;
Jiang, Bo-Le ;
Lu, Guang-Tao ;
Tang, Ji-Liang .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2009, 22 (03) :321-329
[9]   Type III protein secretion systems in bacterial pathogens of animals and plants [J].
Hueck, CJ .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (02) :379-+
[10]   Transcription Factors in Escherichia coli Prefer the Holo Conformation [J].
Itzel Balderas-Martinez, Yalbi ;
Savageau, Michael ;
Salgado, Heladia ;
Perez-Rueda, Ernesto ;
Morett, Enrique ;
Collado-Vides, Julio .
PLOS ONE, 2013, 8 (06)
1234