An updated procedure for zeaxanthin and lutein quantification in corn grains based only in water and ethanol

被引：2

作者：

Carneiro, Ariadne M. ^{[1
]}

Lima, Bruna R. ^{[1
]}

Chibli, Lucas A. ^{[1
]}

Carneiro, Renato L. ^{[2
]}

Funari, Cristiano S. ^{[1
]}

机构：

[1] Sao Paulo State Univ, Coll Agr Sci, Green Biotech Network, Ave Univ 3780, BR-18605525 Sao Paulo, Brazil

[2] Univ Fed Sao Carlos, Dept Chem, Rod Washington Luiz S-N, BR-13565905 Sao Paulo, Brazil

来源：

FOOD CHEMISTRY | 2023年 / 427卷

基金：

巴西圣保罗研究基金会;

关键词：

Sample preparation; Green analytical chemistry; Design of experiments; Zea mays L; UHPLC; LIQUID-CHROMATOGRAPHY; MAIZE;

D O I：

10.1016/j.foodchem.2023.136589

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Corn grains are a major source of both the bioactive carotenoids zeaxanthin and lutein. Current methods to quantify these substances have some disadvantages related to sustainability and sample throughput. This work aimed to develop a green, efficient, rapid, and reproducible analytical method to quantify these xanthophylls in corn grains. Solvents recommended by the CHEM21 solvent selection guide were screened. The extraction by dynamic maceration and separation by ultra-high-performance liquid chromatography were optimized by design of experiments. Then, the entire analytical procedure was validated and compared with procedures used for the same purpose, including an official one, and applied to different corn samples. The proposed method was demonstrated to be greener, equal to or more efficient, faster, and more reproducible than the comparative methods. The extraction step could be scaled up for industrial production of zeaxanthin-and lutein-enriched extracts, as it uses only compatible food grade ethanol and water.

引用

页数：9

共 39 条

[1]

AACC Approved Methods of Analysis, 1999, Method 14-50.01. Determination of Pigments. Approved November 3, V11th, DOI [10.1094/AACCIntMethod-14-50.01, DOI 10.1094/AACCINTMETHOD-14-50.01]

[2] Green extraction techniques in green analytical chemistry [J].

Armenta, Sergio ;

Garrigues, Salvador ;

Esteve-Turrillas, Francesc A. ;

de la Guardia, Miguel .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2019, 116 :248-253

[3] Lutein plus Zeaxanthin and Omega-3 Fatty Acids for Age-Related Macular Degeneration The Age-Related Eye Disease Study 2 (AREDS2) Randomized Clinical Trial [J].

Chew, Emily Y. ;

Clemons, Traci E. ;

SanGiovanni, John Paul ;

Danis, Ronald ;

Ferris, Frederick L., III ;

Elman, Michael ;

Antoszyk, Andrew ;

Ruby, Alan ;

Orth, David ;

Bressler, Susan ;

Fish, Gary ;

Hubbard, Baker ;

Klein, Michael ;

Chandra, Suresh ;

Blodi, Barbara ;

Domalpally, Amitha ;

Friberg, Thomas ;

Wong, Wai ;

Rosenfeld, Philip ;

Agron, Elvira ;

Toth, Cynthia ;

Bernstein, Paul ;

Sperduto, Robert .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2013, 309 (19) :2005-2015

[4] Zea mays L. Grain: Increase in Nutraceutical and Antioxidant Properties Due to Se Fortification in Low and High Water Regimes [J].

D'Amato, Roberto ;

De Feudis, Mauro ;

Guiducci, Marcello ;

Businelli, Daniela .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (25) :7050-7059

[5] Incorporation of zeaxanthin nanoparticles in yogurt: Influence on physicochemical properties, carotenoid stability and sensory analysis [J].

de Campo, Camila ;

Assis, Renato Queiroz ;

da Silva, Medelin Marques ;

Haas Costa, Tania Maria ;

Paese, Karina ;

Guterres, Silvia Staniscuaski ;

Rios, Alessandro de Oliveira ;

Floresa, Simone Hickmann .

FOOD CHEMISTRY, 2019, 301

[6] Screening sweetcorn for enhanced zeaxanthin concentration [J].

Fanning, Kent J. ;

Martin, Ian ;

Wong, Lung ;

Keating, Veronique ;

Pun, Sharon ;

O'Hare, Tim .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2010, 90 (01) :91-96

[7] Reaction of the Phytochemistry Community to Green Chemistry: Insights Obtained Since 1990 [J].

Funari, Cristiano S. ;

Rinaldo, Daniel ;

Bolzani, Vanderlan S. ;

Verpoorte, Robert .

JOURNAL OF NATURAL PRODUCTS, 2023, :440-459

[8] HPLC-EAT (Environmental Assessment Tool): A tool for profiling safety, health and environmental impacts of liquid chromatography methods [J].

Gaber, Yasser ;

Tornvall, Ulrika ;

Kumar, M. A. ;

Amin, Magdy Ali ;

Hatti-Kaul, Rajni .

GREEN CHEMISTRY, 2011, 13 (08) :2021-2025

[9] The 12 principles of green analytical chemistry and the SIGNIFICANCE mnemonic of green analytical practices [J].

Galuszka, Agnieszka ;

Migaszewski, Zdzislaw ;

Namiesnik, Jacek .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2013, 50 :78-84

[10] Method transfer for fast liquid chromatography in pharmaceutical analysis: Application to short columns packed with small particle. Part II: Gradient experiments [J].

Guillarme, Davy ;

Nguyen, Dao T. T. ;

Rudaz, Serge ;

Veuthey, Jean-Luc .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2008, 68 (02) :430-440

← 1 2 3 4 →