3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy

被引：19

作者：

Li, Congsun ^{[1
,2
,3
,4
]}

Wang, Jie ^{[1
,2
,3
,4
]}

Yang, Weinan ^{[1
,2
,3
,4
]}

Yu, Kang ^{[5
]}

Hong, Jianqiao ^{[1
,2
,3
,4
]}

Ji, Xiaoxiao ^{[1
]}

Yao, Minjun ^{[1
,2
,3
,4
]}

Li, Sihao ^{[1
,2
,3
,4
]}

Lu, Jinwei ^{[1
,2
,3
,4
]}

Chen, Yazhou ^{[1
,2
,3
,4
]}

Yan, Shigui ^{[1
,2
,3
,4
]}

Wu, Haobo ^{[1
,2
,3
,4
]}

Ma, Chiyuan ^{[1
,2
,3
,4
]}

Yu, Xiaohua ^{[1
,2
,3
,4
]}

Jiang, Guangyao ^{[1
,2
,3
,4
]}

Liu, An ^{[1
,2
,3
,4
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 2, Dept Orthoped Surg, Sch Med, Hangzhou, Zhejiang, Peoples R China

[2] Zhejiang Univ, Orthoped Res Inst, Hangzhou, Zhejiang, Peoples R China

[3] Key Lab Motor Syst Dis Res & Precis Therapy Zhejia, Hangzhou, Zhejiang, Peoples R China

[4] Clin Res Ctr Motor Syst Dis Zhejiang Prov, Hangzhou, Peoples R China

[5] Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou, Zhejiang, Peoples R China

来源：

JOURNAL OF NANOBIOTECHNOLOGY | 2023年 / 21卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Hydrogel; TDSCs; Tendinopathy; Inflammation; PI3K-AKT pathway; PLATELET-RICH PLASMA; MESENCHYMAL STEM-CELLS; BONE-MARROW; SIGNALING PATHWAY; DIFFERENTIATION; INFLAMMATION; BIOLOGY; ROLES;

D O I：

10.1186/s12951-023-01892-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Long-term chronic inflammation after Achilles tendon injury is critical for tendinopathy. Platelet-rich plasma (PRP) injection, which is a common method for treating tendinopathy, has positive effects on tendon repair. In addition, tendon-derived stem cells (TDSCs), which are stem cells located in tendons, play a major role in maintaining tissue homeostasis and postinjury repair. In this study, injectable gelatine methacryloyl (GelMA) microparticles containing PRP laden with TDSCs (PRP-TDSC-GM) were prepared by a projection-based 3D bioprinting technique. Our results showed that PRP-TDSC-GM could promote tendon differentiation in TDSCs and reduce the inflammatory response by downregulating the PI3K-AKT pathway, thus promoting the structural and functional repair of tendons in vivo.

引用

页数：14

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