3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy

被引：14
作者：
Li, Congsun [1 ,2 ,3 ,4 ]
Wang, Jie [1 ,2 ,3 ,4 ]
Yang, Weinan [1 ,2 ,3 ,4 ]
Yu, Kang [5 ]
Hong, Jianqiao [1 ,2 ,3 ,4 ]
Ji, Xiaoxiao [1 ]
Yao, Minjun [1 ,2 ,3 ,4 ]
Li, Sihao [1 ,2 ,3 ,4 ]
Lu, Jinwei [1 ,2 ,3 ,4 ]
Chen, Yazhou [1 ,2 ,3 ,4 ]
Yan, Shigui [1 ,2 ,3 ,4 ]
Wu, Haobo [1 ,2 ,3 ,4 ]
Ma, Chiyuan [1 ,2 ,3 ,4 ]
Yu, Xiaohua [1 ,2 ,3 ,4 ]
Jiang, Guangyao [1 ,2 ,3 ,4 ]
Liu, An [1 ,2 ,3 ,4 ]
机构：
[1] Zhejiang Univ, Affiliated Hosp 2, Dept Orthoped Surg, Sch Med, Hangzhou, Zhejiang, Peoples R China
[2] Zhejiang Univ, Orthoped Res Inst, Hangzhou, Zhejiang, Peoples R China
[3] Key Lab Motor Syst Dis Res & Precis Therapy Zhejia, Hangzhou, Zhejiang, Peoples R China
[4] Clin Res Ctr Motor Syst Dis Zhejiang Prov, Hangzhou, Peoples R China
[5] Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou, Zhejiang, Peoples R China
来源：
JOURNAL OF NANOBIOTECHNOLOGY | 2023年 / 21卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Hydrogel; TDSCs; Tendinopathy; Inflammation; PI3K-AKT pathway; PLATELET-RICH PLASMA; MESENCHYMAL STEM-CELLS; BONE-MARROW; SIGNALING PATHWAY; DIFFERENTIATION; INFLAMMATION; BIOLOGY; ROLES;
D O I：
10.1186/s12951-023-01892-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Long-term chronic inflammation after Achilles tendon injury is critical for tendinopathy. Platelet-rich plasma (PRP) injection, which is a common method for treating tendinopathy, has positive effects on tendon repair. In addition, tendon-derived stem cells (TDSCs), which are stem cells located in tendons, play a major role in maintaining tissue homeostasis and postinjury repair. In this study, injectable gelatine methacryloyl (GelMA) microparticles containing PRP laden with TDSCs (PRP-TDSC-GM) were prepared by a projection-based 3D bioprinting technique. Our results showed that PRP-TDSC-GM could promote tendon differentiation in TDSCs and reduce the inflammatory response by downregulating the PI3K-AKT pathway, thus promoting the structural and functional repair of tendons in vivo.
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页数：14
相关论文
共 55 条
  • [1] BIOACTIVE HYDROGELS Lighting the way
    Alge, Daniel L.
    Anseth, Kristi S.
    [J]. NATURE MATERIALS, 2013, 12 (11) : 950 - 952
  • [2] Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche
    Bi, Yanming
    Ehirchiou, Driss
    Kilts, Tina M.
    Inkson, Colette A.
    Embree, Mildred C.
    Sonoyama, Wataru
    Li, Li
    Leet, Arabella I.
    Seo, Byoung-Moo
    Zhang, Li
    Shi, Songtao
    Young, Marian F.
    [J]. NATURE MEDICINE, 2007, 13 (10) : 1219 - 1227
  • [3] Systemic corticosteroids improve tendon healing when given after the early inflammatory phase
    Blomgran, Parmis
    Hammerman, Malin
    Aspenberg, Per
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [4] Celecoxib, Beyond Anti-inflammation, Alleviates Tendon-Derived Stem Cell Senescence in Degenerative Rotator Cuff Tendinopathy
    Cai, Zhuochang
    Zhang, Yao
    Liu, Shen
    Liu, Xudong
    [J]. AMERICAN JOURNAL OF SPORTS MEDICINE, 2022, 50 (09) : 2488 - 2496
  • [5] Platelet-rich plasma pretreatment protects anterior cruciate ligament fibroblasts correlated with PI3K-Akt-mTOR pathway under hypoxia condition
    Cao, Yanwei
    Li, Yue
    Fu, Sai Chuen
    Shen, Jiewei
    Zhang, Hui
    Jiang, Chunyan
    Yung, Patrick Shu-Hang
    [J]. JOURNAL OF ORTHOPAEDIC TRANSLATION, 2022, 34 : 102 - 112
  • [6] Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial
    Carvalho, Armando de Mattos
    Badial, Peres Ramos
    Cisneros Alvarez, Luis Emiliano
    Miluzzi Yamada, Ana Lucia
    Borges, Alexandre Secorun
    Deffune, Elenice
    Hussni, Carlos Alberto
    Garcia Alves, Ana Liz
    [J]. STEM CELL RESEARCH & THERAPY, 2013, 4
  • [7] Chen J, 2013, TISSUE ENG PART B-RE, V19, P516, DOI [10.1089/ten.teb.2012.0672, 10.1089/ten.TEB.2012.0672]
  • [8] Tendon healing in presence of chronic low-level inflammation: a systematic review
    Chisari, Emanuele
    Rehak, Laura
    Khan, Wasim S.
    Maffulli, Nicola
    [J]. BRITISH MEDICAL BULLETIN, 2019, 132 (01) : 97 - 116
  • [9] Activation of AKT-mTOR Signaling Directs Tenogenesis of Mesenchymal Stem Cells
    Cong, Xiao Xia
    Rao, Xi Sheng
    Lin, Jun Xin
    Liu, Xiao Ceng
    Zhang, Guang An
    Gao, Xiu Kui
    He, Min Yi
    Shen, Wei Liang
    Fan, Wei
    Pioletti, Dominique
    Zheng, Li Ling
    Liu, Huan Huan
    Yin, Zi
    Low, Boon Chuan
    Schweitzer, Ronen
    Ouyang, Hongwei
    Chen, Xiao
    Zhou, Yi Ting
    [J]. STEM CELLS, 2018, 36 (04) : 527 - 539
  • [10] Chronic inflammation is a feature of Achilles tendinopathy and rupture
    Dakin, Stephanie Georgina
    Newton, Julia
    Martinez, Fernando O.
    Hedley, Robert
    Gwilym, Stephen
    Jones, Natasha
    Reid, Hamish A. B.
    Wood, Simon
    Wells, Graham
    Appleton, Louise
    Wheway, Kim
    Watkins, Bridget
    Carr, Andrew Jonathan
    [J]. BRITISH JOURNAL OF SPORTS MEDICINE, 2018, 52 (06) : 359 - 367
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