Polysaccharides from natural Cordyceps sinensis attenuated dextran sodium sulfate-induced colitis in C57BL/6J mice

被引:1
|
作者
Chen, Shuping [1 ,2 ]
Wang, Junqiao [2 ]
Dong, Nan [2 ]
Fang, Qiuyue [2 ]
Zhang, Yanli [2 ]
Chen, Chunhua [2 ]
Cui, Steve W. W. [2 ,3 ]
Nie, Shaoping [2 ]
机构
[1] Jiangxi Agr Univ, Jiangxi Key Lab Nat Prod & Funct Foods, Nanchang 330045, Peoples R China
[2] Nanchang Univ, State Key Lab Food Sci & Technol, Key Lab Bioact Polysaccharides Jiangxi Prov, China Canada Joint Lab Food Sci & Technol Nanchang, Nanchang 330047, Peoples R China
[3] Agr & Agri Food Canada, Guelph Res & Dev Ctr, 93 Stone Rd West, Guelph, ON N1G 5C9, Canada
基金
中国国家自然科学基金;
关键词
ULCERATIVE-COLITIS; GUT MICROBIOTA; INFLAMMATORY RESPONSES; ANTIOXIDANT ACTIVITY; BARRIER; EXPRESSION; IMMUNITY; CELLS; GPR41;
D O I
10.1039/d2fo02555h
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As potential candidates for treating ulcerative colitis (UC), polysaccharides have been attracting extensive interest in recent years. Cordyceps sinensis (C. sinensis) is a kind of traditional Chinese edible food, and its polysaccharide fractions have been found to be effective in regulating immunity and protecting the kidneys. To determine the potential function of polysaccharides from natural C. sinensis on UC, their effects in terms of histological, serological, biochemical, and immunological aspects on dextran sulphate sodium (DSS)-induced colitis mice model were investigated. Results showed that the polysaccharides significantly alleviated colitis by increasing the colon length, alleviating colon tissue damage, and inhibiting the activation of the NF-kappa B pathway. In addition, polysaccharides reduced the contents of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in the serum, increased the number of goblet cells, and improved the expression of intestinal tight junction proteins (Occludin and Claudin-1). They also evidently enhanced the formation of IgA-secretory cells and sIgA contents. Furthermore, the polysaccharides modulated the gut microbiota by decreasing the relative abundance of Bilophila and increasing the relative abundance of Dehalobacterium, Coprococcus, Oscillospira, and Desulfovibrio, which is accompanied by an increase in the short chain fatty acids' (SCFAs) concentrations in cecal contents. These results suggested that C. sinensis polysaccharides possessed promising intervening effects on experimental acute UC in mice.
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页码:720 / 733
页数:15
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