Green Synthesis of Zinc Oxide Nanoparticles from Althaea officinalis Flower Extract Coated with Chitosan for Potential Healing Effects on Diabetic Wounds by Inhibiting TNF-α and IL-6/IL-1β Signaling Pathways

被引：20

作者：

Elhabal, Sammar Fathy ^{[1
]}

Abdelaal, Nashwa ^{[2
]}

Al-Zuhairy, Saeed Abdul Kareem Saeed ^{[3
]}

Elrefai, Mohamed Fathi Mohamed ^{[4
,5
]}

Hamdan, Ahmed Mohsen Elsaid ^{[6
]}

Khalifa, Mohamed Mansour ^{[7
]}

Hababeh, Sandra ^{[8
]}

Khasawneh, Mohammad Ahmad ^{[9
]}

Khamis, Gehad M. ^{[10
]}

Nelson, Jakline ^{[11
]}

Mohie, Passant M. ^{[1
]}

Gad, Rania A. ^{[1
,2
,12
]}

Rizk, Amira ^{[1
,3
,13
]}

Kabil, Soad L. ^{[1
,4
,14
]}

El-Ashery, Mohamed Kandeel ^{[1
,5
,6
,15
,16
]}

Jasti, Bhaskara R. ^{[17
]}

Elzohairy, Nahla A. ^{[18
,19
]}

Elnawawy, Tayseer ^{[20
]}

Hassan, Fatma E. ^{[21
,22
]}

El-Nabarawi, Mohamed ^{[23
]}

机构：

[1] Modern Univ Technol & Informat MTI, Fac Pharm, Dept Pharmaceut & Ind Pharm, Cairo, Egypt

[2] Baylor Coll Med, Dept Integrat Physiol, Houston, TX USA

[3] Kut Univ Coll, Dept Pharm, Kut, Iraq

[4] Hashemite Univ, Fac Med, Dept Anat Hist Physiol & Biochem, Zarqa, Jordan

[5] Ain Shams Univ, Fac Med, Dept Anat & Embryol, Cairo, Egypt

[6] Univ Tabuk, Fac Pharm, Dept Pharm Practice, Tabuk, Saudi Arabia

[7] Cairo Univ, Fac Med, Dept Human Physiol, Cairo, Egypt

[8] King Saud Univ, Coll Pharm, Dept Pharmaceut, Riyadh, Saudi Arabia

[9] UAE Univ, Coll Sci, Dept Chem, Al Ain, U Arab Emirates

[10] Alexandria Univ, Fac Med, Dept Clin Pharmacol, Alexandria, Egypt

[11] Beni Suef Univ, Fac Pharm, Dept Microbiol & Immunol, Bani Suwayf, Egypt

[12] Nahda Univ, Dept Pharmacol & Toxicol, Fac Pharm, Bani Suwayf, Egypt

[13] Tanta Univ, Fac Agr, Dept Food Sci & Technol, Tanta, Egypt

[14] Zagazig Univ, Fac Med, Dept Clin Pharmacol, Zagazig, Egypt

[15] Cairo Univ, Fac Pharm, Pharmaceut Chem Dept, Cairo, Egypt

[16] King Salman Int Univ, Fac Pharm, Med Chem Dept, South Sinai, Egypt

[17] Univ Pacific, Thomas J Long Sch Pharm, Dept Pharmaceut & Med Chem, Stockton, CA USA

[18] Air Force Specialized Hosp, Cairo, Egypt

[19] Modern Univ Technol & Informat MTI, Fac Pharm, Dept Microbiol & Immunol, Cairo, Egypt

[20] Egyptian Drug Author, Dept Pharmaceut, Cairo, Egypt

[21] Cairo Univ, Fac Med, Med Physiol Dept, Giza, Egypt

[22] Batterjee Med Coll, Dept Microbiol, Gen Med Practice Program, Jeddah, Saudi Arabia

[23] Cairo Univ, Fac Pharm, Dept Pharmaceut & Ind Pharm, Cairo, Egypt

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2024年 / 19卷

关键词：

wound healing; antimicrobial; antioxidant; Staphylococcus aureus; Bacillus subtilis; Escherichia coli; Pseudomonas aeruginosa; chitosan; wound concentration; wound incision; AERIAL PARTS; IN-VITRO; DELIVERY; VIVO;

D O I：

10.2147/IJN.S455270

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Background: Diabetes Mellitus is a multisystem chronic pandemic, wound inflammation, and healing are still major issues for diabetic patients who may suffer from ulcers, gangrene, and other wounds from uncontrolled chronic hyperglycemia. Marshmallows or Althaea officinalis (A.O.) contain bioactive compounds such as flavonoids and phenolics that support wound healing via antioxidant, anti-inflammatory, and antibacterial properties. Our study aimed to develop a combination of eco-friendly formulations of green synthesis of ZnO-NPs by Althaea officinalis extract and further incorporate them into 2% chitosan (CS) gel. Method and Results: First, develop eco-friendly green Zinc Oxide Nanoparticles (ZnO-NPs) and incorporate them into a 2% chitosan (CS) gel. In -vitro study performed by UV -visible spectrum analysis showed a sharp peak at 390 nm, and Energy -dispersive X-ray (EDX) spectrometry showed a peak of zinc and oxygen. Besides, Fourier transforms infrared (FTIR) was used to qualitatively validate biosynthesized ZnO-NPs, and transmission electron microscope (TEM) showed spherical nanoparticles with mean sizes of 76 nm and Zeta potential +30mV. The antibacterial potential of A.O.-ZnO-NPs-Cs was examined by the diffusion agar method against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Based on the zone of inhibition and minimal inhibitory indices (MIC). In addition, an in-silico study investigated the binding affinity of A.O. major components to the expected biological targets that may aid wound healing. Althaea Officinalis, A.O-ZnO-NPs group showed reduced downregulation of IL -6, IL-1 beta, and TNF-alpha and increased IL -10 levels compared to the control group signaling pathway expression levels confirming the improved anti-inflammatory effect of the self -assembly method. In -vivo study and histopathological analysis revealed the superiority of the nanoparticles in reducing signs of inflammation and wound incision in rat models. Conclusion: These biocompatible green zinc oxide nanoparticles, by using Althaea Officinalis chitosan gel ensure an excellent new therapeutic approach for quickening diabetic wound healing.

引用

页码：3045 / 3070

页数：26

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