Single-cell RNA sequencing comparison of the human metastatic prostate spine tumor microenvironment

被引：0

作者：

Nguyen, Annee D. ^{[1
]}

Haines, Corinne ^{[2
]}

Price, Meghan J. ^{[3
]}

Dalton, Tara E. ^{[1
]}

Baeta, Cesar D. ^{[4
]}

Hockenberry, Harrison A. ^{[1
]}

Goodwin, C. Rory ^{[1
]}

机构：

[1] Duke Univ, Dept Neurosurg, Med Ctr, 200 Trent Dr, Durham, NC 27710 USA

[2] Ohio State Univ, Dept Mol Genet, 1060 Carmack Rd, Columbus, OH 43210 USA

[3] Johns Hopkins Univ Hosp, Dept Med, 1800 Orleans St, Baltimore, MD 21287 USA

[4] Stanford Univ, Ctr Populat Hlth Sci, 1701 Page Mill Rd, Palo Alto, CA 94304 USA

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 01期

关键词：

Cancer; RNA-seq; Sequencing; Single Cell;

D O I：

10.1016/j.xpro.2023.102805

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Spinal column tumors can be difficult to process for single -cell omic studies, given the heterogeneity in tissue. Here, we present a protocol for operating room-tobenchtop single -cell processing of clinical specimens from a prostate cancer patient. We describe steps for sample homogenization, red blood cell lysis, cryopreservation, and single -cell sequencing analysis. This protocol can be used to identify prognostic markers and therapeutic targets for patients with osseous spine metastases and better inform eligibility for clinical trials.

引用

页数：16

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