Ropivacaine inhibits proliferation and invasion and promotes apoptosis and autophagy in bladder cancer cells via inhibiting PI3K/AKT pathway
被引:10
作者:
Qiao, Hui
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机构:
Capital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R ChinaCapital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
Qiao, Hui
[1
]
Zhang, Wei
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机构:
Zhengzhou Univ, Henan Prov Peoples Hosp, Dept Anesthesiol & Perioperat Med, Peoples Hosp, 7 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R ChinaCapital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
Zhang, Wei
[2
]
Liu, Pengfei
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h-index: 0
机构:
Capital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R ChinaCapital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
Liu, Pengfei
[1
]
Zhu, Ruilou
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h-index: 0
机构:
Zhengzhou Univ, Henan Prov Peoples Hosp, Dept Anesthesiol & Perioperat Med, Peoples Hosp, 7 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R ChinaCapital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
Zhu, Ruilou
[2
]
Zhang, Jing
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机构:
Capital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R ChinaCapital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
Zhang, Jing
[1
]
Gao, Jing
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机构:
Capital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R ChinaCapital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
Gao, Jing
[1
]
Li, Tianzuo
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Capital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R ChinaCapital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
Li, Tianzuo
[1
]
Zhang, Jiaqiang
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机构:
Zhengzhou Univ, Henan Prov Peoples Hosp, Dept Anesthesiol & Perioperat Med, Peoples Hosp, 7 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R ChinaCapital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
Zhang, Jiaqiang
[2
]
机构:
[1] Capital Med Univ, Beijing Shijitan Hosp, Dept Anesthesiol, Beijing, Peoples R China
apoptosis;
autophagy;
bladder cancer;
local anesthetics;
PI3K;
AKT signal pathway;
D O I:
10.1002/jbt.23233
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Application of a certain concentration of local anesthetics during tumor resection inhibits the progression of tumor. The effects of ropivacaine in bladder cancer (BC) have never been explored. We explored the effects of ropivacaine on the progression of BC in vitro and in vivo. CCK8 assay and EDU staining was conducted to examine cell proliferation. Flow cytometry and transwell assay were performed to evaluate apoptosis and invasion, respectively. Expression of light chain 3 (LC3) was observed through immunofluorescence. Furthermore, the xenograft tumor model of BC was built to detect the effects of ropivacaine in vivo. IHC and TUNEL assay were conducted to detect cell proliferation and apoptosis in vivo. Ropivacaine inhibited the proliferation of T24 and 5639 cells with the 50% inhibitory concentration (IC50) of 20.08 and 31.86 mu M, respectively. Ropivacaine suppressed the invasion ability and induces the apoptosis of cells. Besides, ropivacaine triggers obvious autophagy in BC cells. Moreover, ropivacaine blocks the PI3K/AKT signal pathway in BC cells. The impact of ropivacaine on cell viability, motility, and autophagy was reversed by 740 Y-P, the activator of PI3K/AKT signal pathway. The in vivo experiments demonstrated that ropivacaine inhibited the proliferation and mobility of BC. Ropivacaine has anti-carcinoma effects in BC via inactivating PI3K/AKT pathway, providing a new theoretical reference for the use of local anesthetics in the treatment of BC.
机构:
Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Bao, Jinfang
Shi, Yingfeng
论文数: 0引用数: 0
h-index: 0
机构:
Tongji Univ, Shanghai East Hosp, Dept Nephrol, Sch Med, Shanghai 200120, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Shi, Yingfeng
Tao, Min
论文数: 0引用数: 0
h-index: 0
机构:
Tongji Univ, Shanghai East Hosp, Dept Nephrol, Sch Med, Shanghai 200120, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Tao, Min
Liu, Na
论文数: 0引用数: 0
h-index: 0
机构:
Tongji Univ, Shanghai East Hosp, Dept Nephrol, Sch Med, Shanghai 200120, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Liu, Na
Zhuang, Shougang
论文数: 0引用数: 0
h-index: 0
机构:
Tongji Univ, Shanghai East Hosp, Dept Nephrol, Sch Med, Shanghai 200120, Peoples R China
Brown Univ, Rhode Isl Hosp, Dept Med, Providence, RI 02903 USA
Brown Univ, Alpert Med Sch, Providence, RI 02912 USAShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Zhuang, Shougang
Yuan, Weijie
论文数: 0引用数: 0
h-index: 0
机构:
Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
机构:
All India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, IndiaAll India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, India
Bharati, Sachidanand Jee
Chowdhury, Tumul
论文数: 0引用数: 0
h-index: 0
机构:
Univ Manitoba, Dept Anesthesiol & Perioperat Med, Winnipeg, MB, CanadaAll India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, India
Chowdhury, Tumul
Bergese, Sergio D.
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Wexner Med Ctr, Dept Anesthesiol & Neurol Surg, Columbus, OH 43210 USAAll India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, India
Bergese, Sergio D.
Ghosh, Subhamay
论文数: 0引用数: 0
h-index: 0
机构:
Univ Manitoba, Dept Anesthesiol & Perioperat Med, Winnipeg, MB, Canada
Univ Pecs, Dept Anaesthesiol & Intens Therapy, Pecs, HungaryAll India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, India
机构:
Penn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Penn State Univ, Coll Med, Penn State Hershey Canc Inst, Hershey, PA 17033 USA
Milton S Hershey Med Ctr, Hershey, PA 17033 USAPenn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Cheng, Yan
Ren, Xingcong
论文数: 0引用数: 0
h-index: 0
机构:
Penn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Penn State Univ, Coll Med, Penn State Hershey Canc Inst, Hershey, PA 17033 USA
Milton S Hershey Med Ctr, Hershey, PA 17033 USAPenn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Ren, Xingcong
Hait, William N.
论文数: 0引用数: 0
h-index: 0
机构:
Janssen Res & Dev, Raritan, NJ USAPenn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Hait, William N.
Yang, Jin-Ming
论文数: 0引用数: 0
h-index: 0
机构:
Penn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Penn State Univ, Coll Med, Penn State Hershey Canc Inst, Hershey, PA 17033 USA
Milton S Hershey Med Ctr, Hershey, PA 17033 USAPenn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
机构:
Imperial Coll London Chelsea, Dept Anaesthesia, London, England
Westminster Med Sch & Hosp, London, EnglandImperial Coll London Chelsea, Dept Anaesthesia, London, England
Ciechanowicz, S. J.
Ma, D.
论文数: 0引用数: 0
h-index: 0
机构:
Westminster Med Sch & Hosp, London, England
Imperial Coll London Chelsea, Dept Surg & Canc, Anaesthet Pain Med & Intens Care, Anaesthesia Res, London, EnglandImperial Coll London Chelsea, Dept Anaesthesia, London, England
机构:
Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Bao, Jinfang
Shi, Yingfeng
论文数: 0引用数: 0
h-index: 0
机构:
Tongji Univ, Shanghai East Hosp, Dept Nephrol, Sch Med, Shanghai 200120, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Shi, Yingfeng
Tao, Min
论文数: 0引用数: 0
h-index: 0
机构:
Tongji Univ, Shanghai East Hosp, Dept Nephrol, Sch Med, Shanghai 200120, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Tao, Min
Liu, Na
论文数: 0引用数: 0
h-index: 0
机构:
Tongji Univ, Shanghai East Hosp, Dept Nephrol, Sch Med, Shanghai 200120, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Liu, Na
Zhuang, Shougang
论文数: 0引用数: 0
h-index: 0
机构:
Tongji Univ, Shanghai East Hosp, Dept Nephrol, Sch Med, Shanghai 200120, Peoples R China
Brown Univ, Rhode Isl Hosp, Dept Med, Providence, RI 02903 USA
Brown Univ, Alpert Med Sch, Providence, RI 02912 USAShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
Zhuang, Shougang
Yuan, Weijie
论文数: 0引用数: 0
h-index: 0
机构:
Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R ChinaShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Nephrol, Sch Med, Shanghai 200080, Peoples R China
机构:
All India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, IndiaAll India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, India
Bharati, Sachidanand Jee
Chowdhury, Tumul
论文数: 0引用数: 0
h-index: 0
机构:
Univ Manitoba, Dept Anesthesiol & Perioperat Med, Winnipeg, MB, CanadaAll India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, India
Chowdhury, Tumul
Bergese, Sergio D.
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Wexner Med Ctr, Dept Anesthesiol & Neurol Surg, Columbus, OH 43210 USAAll India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, India
Bergese, Sergio D.
Ghosh, Subhamay
论文数: 0引用数: 0
h-index: 0
机构:
Univ Manitoba, Dept Anesthesiol & Perioperat Med, Winnipeg, MB, Canada
Univ Pecs, Dept Anaesthesiol & Intens Therapy, Pecs, HungaryAll India Inst Med Sci, Dr BRAIRCH, Dept Anesthesiol, New Delhi, India
机构:
Penn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Penn State Univ, Coll Med, Penn State Hershey Canc Inst, Hershey, PA 17033 USA
Milton S Hershey Med Ctr, Hershey, PA 17033 USAPenn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Cheng, Yan
Ren, Xingcong
论文数: 0引用数: 0
h-index: 0
机构:
Penn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Penn State Univ, Coll Med, Penn State Hershey Canc Inst, Hershey, PA 17033 USA
Milton S Hershey Med Ctr, Hershey, PA 17033 USAPenn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Ren, Xingcong
Hait, William N.
论文数: 0引用数: 0
h-index: 0
机构:
Janssen Res & Dev, Raritan, NJ USAPenn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Hait, William N.
Yang, Jin-Ming
论文数: 0引用数: 0
h-index: 0
机构:
Penn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
Penn State Univ, Coll Med, Penn State Hershey Canc Inst, Hershey, PA 17033 USA
Milton S Hershey Med Ctr, Hershey, PA 17033 USAPenn State Univ, Coll Med, Dept Pharmacol, Hershey, PA 17033 USA
机构:
Imperial Coll London Chelsea, Dept Anaesthesia, London, England
Westminster Med Sch & Hosp, London, EnglandImperial Coll London Chelsea, Dept Anaesthesia, London, England
Ciechanowicz, S. J.
Ma, D.
论文数: 0引用数: 0
h-index: 0
机构:
Westminster Med Sch & Hosp, London, England
Imperial Coll London Chelsea, Dept Surg & Canc, Anaesthet Pain Med & Intens Care, Anaesthesia Res, London, EnglandImperial Coll London Chelsea, Dept Anaesthesia, London, England