The chemistry of next-generation sequencing

被引：38

作者：

Rodriguez, Raphael ^{[1
]}

Krishnan, Yamuna ^{[2
,3
,4
]}

机构：

[1] PSL Res Univ, Equipe Labellisee Ligue Canc, INSERM, Inst Curie,CNRS, Paris, France

[2] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

[3] Univ Chicago, Neurosci Inst, Chicago, IL 60637 USA

[4] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA

来源：

NATURE BIOTECHNOLOGY | 2023年 / 41卷 / 12期

基金：

欧洲研究理事会;

关键词：

DNA; FLUORESCENT;

D O I：

10.1038/s41587-023-01986-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The first large genome fully sequenced by next-generation sequencing (NGS) was that of a bacteriophage using sequencing by synthesis (SBS) as a paradigm. SBS in NGS is underpinned by 'reversible-terminator chemistry'. To grow from proof of concept to being both affordable and practical, SBS needed to overcome a series of challenges, each of which required the invention of new chemistries. These included the design and synthesis of unnatural deoxynucleotide triphosphates (dNTPs), engineering a suitable polymerase, a new surface chemistry and an ingenious molecular solution to neutralize copying errors inherent to all polymerases. In this historical Perspective, we discuss how NGS was developed from Sanger sequencing, highlighting the chemistry behind this technology, which has impacted biology in unprecedented ways. A historical perspective on how next-generation sequencing chemistry was developed.

引用

页码：1709 / 1715

页数：7

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