Comprehensive Characterization of a Novel Bacteriophage, vB_VhaS_MAG7 against a Fish Pathogenic Strain of Vibrio harveyi and Its In Vivo Efficacy in Phage Therapy Trials

被引：7

作者：

Droubogiannis, Stavros ^{[1
]}

Pavlidi, Lydia ^{[1
]}

Skliros, Dimitrios ^{[2
]}

Flemetakis, Emmanouil ^{[2
]}

Katharios, Pantelis ^{[1
]}

机构：

[1] Hellen Ctr Marine Res, Inst Marine Biol Biotechnol & Aquaculture, Iraklion 71500, Greece

[2] Agr Univ Athens, Sch Food Biotechnol & Dev, Dept Biotechnol, Lab Mol Biol, Athens 11855, Greece

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 09期

关键词：

Vibrio harveyi; aquaculture; phage therapy; gilthead seabream; COMPLETE GENOME SEQUENCE; MARINE FISH; DATABASE;

D O I：

10.3390/ijms24098200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vibrio harveyi, a significant opportunistic marine pathogen, has been a challenge to the aquaculture industry, leading to severe economical and production losses. Phage therapy has been an auspicious approach in controlling such bacterial infections in the era of antimicrobial resistance. In this study, we isolated and fully characterized a novel strain-specific phage, vB_VhaS_MAG7, which infects V. harveyi MM46, and tested its efficacy as a therapeutic agent in challenged gilthead seabream larvae. vB_VhaS_MAG7 is a tailed bacteriophage with a double-stranded DNA of 49,315 bp. No genes linked with virulence or antibiotic resistance were harbored in the genome. The phage had a remarkably large burst size of 1393 PFU cell(-1) and showed strong lytic ability in in vitro assays. When applied in phage therapy trials in challenged gilthead seabream larvae, vB_VhaS_MAG7 was capable of improving the survival of the larvae up to 20%. Due to its distinct features and safety, vB_VhaS_MAG7 is considered a suitable candidate for applied phage therapy.

引用

页数：17