Elucidation of the Anticancer Mechanism of Durian Fruit (Durio zibethinus) Pulp Extract in Human Leukemia (HL-60) Cancer Cells

被引:3
|
作者
Abdul Rahman, Mohamad Sitheek [1 ]
Kanakarajan, Sivakumari [1 ]
Selvaraj, Rajesh [1 ]
Kamalanathan, Ashok [1 ]
Fatima, Sabiha [2 ]
Abudawood, Manal [2 ]
Siddiqi, Nikhat J. [3 ]
Alanazi, Humidah [3 ]
Sharma, Bechan [4 ]
de Lourdes Pereira, Maria [5 ]
机构
[1] Presidency Coll, Dept Zool, Chennai 600005, India
[2] King Saud Univ, Coll Appl Med Sci, Dept Clin Lab Sci, Riyadh 11433, Saudi Arabia
[3] King Saud Univ, Coll Sci, Dept Biochem, Riyadh 11495, Saudi Arabia
[4] Univ Allahabad, Fac Sci, Dept Biochem, Allahabad 211002, India
[5] Univ Aveiro, Aveiro Inst Mat, Dept Med Sci, CICECO, P-3810193 Aveiro, Portugal
关键词
Durio zibethinus; anticancer activity; apoptosis; DNA fragmentation; cell cycle; CYCLE; DNA; ACTIVATION; FLAVONOIDS; APOPTOSIS; PATHWAY; ARREST;
D O I
10.3390/nu15102417
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Durian (Durio zibethinus L.) grows widely in Southeast Asia. The pulp of the durian fruit contains carbohydrates, proteins, lipids, fibers, various vitamins, minerals, and fatty acids. This study was carried out to elucidate the anticancer mechanism of action of the methanolic extract of the fruit of Durio zibethinus (D. zibethinus) on human leukemia (HL-60) cells. The methanolic extract of D. zibethinus fruits exhibited its anticancer effect on HL-60 cells by inducing DNA damage and apoptosis. The DNA damage was confirmed by comet and DNA fragmentation assays. The methanolic extract of D. zibethinus fruits has been shown to cause cell cycle arrest in HL-60 cells during the S phase and G2/M phase. Additionally, the methanolic extract caused induction of the apoptotic pathway in the HL-60 cell line. This was confirmed by increased expression in pro-apoptotic proteins, viz., Bax protein expression, and a substantial reduction (p < 0.001) in anti-apoptotic proteins, viz., Bcl-2 and Bcl-xL expressions. Therefore, this study confirms that the methanolic extract of D. zibethinus exerts its anticancer effects on the HL-60 cell line, causing cell cycle arrest and induction of apoptosis by an intrinsic mechanism.
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页数:12
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